Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor

Tuberculosis (TB) is a major threat to human health due to its increasing global epidemiology and emerging drug-resistant strains. While traditional knowledge (TK) could be a tool for development of novel TB treatment, TK itself is at verge of loss due to modernization and deforestration. The object...

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Main Author:	Sabran, Siti Fatimah
Format:	Thesis
Language:	English English English
Published:	2016
Subjects:	RS Pharmacy and materia medica RS153-441 Materia medica
Online Access:	http://eprints.uthm.edu.my/10042/2/24p%20SITI%20FATIMAH%20SABRAN.pdf http://eprints.uthm.edu.my/10042/1/SITI%20FATIMAH%20SABRAN%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/10042/3/SITI%20FATIMAH%20SABRAN%20WATERMARK.pdf
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spelling	my-uthm-ep.100422023-10-01T07:04:33Z Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor 2016-08 Sabran, Siti Fatimah RS Pharmacy and materia medica RS153-441 Materia medica Tuberculosis (TB) is a major threat to human health due to its increasing global epidemiology and emerging drug-resistant strains. While traditional knowledge (TK) could be a tool for development of novel TB treatment, TK itself is at verge of loss due to modernization and deforestration. The objectives of the study were to document the TK of medicinal plants used for the treatment of TB and its related symptoms as practiced by the Jakun community ofKampung Peta, situated in Taman Negara Johor Endau Rompin; to investigate the antimycobacterial activity and mechanism of action of the selected medicinal plants extracts; and to profile their major phytochemical constituents. Documentation of TK were analyzed qualitatively from semi-structured interviews. Among 23 species documented, water and organic crude extracts of selected plants were evaluated for their antimycobacterial activity using agar disk diffusion, resazurin microplate assay, and agar plate assay against Mycobacterium smegmatis. Upon treatment with the active crude extracts, mechanism of action was investigated via time-kill analysis, leakage of compounds absorbing at 280 nm, field emission-scanning electron microscopy, and 2D proteomic analysis. Findings showed that ethyl acetate extract of Thottea grandifl.ora displayed the largest inhibition zone (DIZ= 14.92 ± 0.86 mm). Hexane extracts of Dipteroca,pus sublamellatus and Tetracera macrophylla showed the lowest minimum inhibition concentration (MIC= 0.78 mg/mL). Ethyl acetate extract of T macrophylla, and both hexane extracts of D. sublamellatus and T macrophylla showed the lowest minimum bactericidal concentration (MBC= 3.13 mg/mL). At 4-fold of MIC, ethyi acetate extract of T macrophylla killed the bacterial cell within 8 hours of treatment by multitarget mechanisms such as inhibition of protein and cell wall synthesi, and distruption of metabolic processes. Using gas chromatography- mass spectrometry (GC-MS) analysis, pyrazole and phenanthroline derivatives were detectes moss among the majorconstituents that potentially contribute to the antimeoheaded activity. The result scientifically validated the plants used in Jakun traditionaI medicine,displayed promising therapeutic properties and further studies in this direction world head to the get antimycobacterial agents 2016-08 Thesis http://eprints.uthm.edu.my/10042/ http://eprints.uthm.edu.my/10042/2/24p%20SITI%20FATIMAH%20SABRAN.pdf text en public http://eprints.uthm.edu.my/10042/1/SITI%20FATIMAH%20SABRAN%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/10042/3/SITI%20FATIMAH%20SABRAN%20WATERMARK.pdf text en validuser phd doctoral Universiti Tun Hussein Onn Malaysia Fakulti Sains, Teknologi dan Pembangunan Insan
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	RS Pharmacy and materia medica RS153-441 Materia medica
spellingShingle	RS Pharmacy and materia medica RS153-441 Materia medica Sabran, Siti Fatimah Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor
description	Tuberculosis (TB) is a major threat to human health due to its increasing global epidemiology and emerging drug-resistant strains. While traditional knowledge (TK) could be a tool for development of novel TB treatment, TK itself is at verge of loss due to modernization and deforestration. The objectives of the study were to document the TK of medicinal plants used for the treatment of TB and its related symptoms as practiced by the Jakun community ofKampung Peta, situated in Taman Negara Johor Endau Rompin; to investigate the antimycobacterial activity and mechanism of action of the selected medicinal plants extracts; and to profile their major phytochemical constituents. Documentation of TK were analyzed qualitatively from semi-structured interviews. Among 23 species documented, water and organic crude extracts of selected plants were evaluated for their antimycobacterial activity using agar disk diffusion, resazurin microplate assay, and agar plate assay against Mycobacterium smegmatis. Upon treatment with the active crude extracts, mechanism of action was investigated via time-kill analysis, leakage of compounds absorbing at 280 nm, field emission-scanning electron microscopy, and 2D proteomic analysis. Findings showed that ethyl acetate extract of Thottea grandifl.ora displayed the largest inhibition zone (DIZ= 14.92 ± 0.86 mm). Hexane extracts of Dipteroca,pus sublamellatus and Tetracera macrophylla showed the lowest minimum inhibition concentration (MIC= 0.78 mg/mL). Ethyl acetate extract of T macrophylla, and both hexane extracts of D. sublamellatus and T macrophylla showed the lowest minimum bactericidal concentration (MBC= 3.13 mg/mL). At 4-fold of MIC, ethyi acetate extract of T macrophylla killed the bacterial cell within 8 hours of treatment by multitarget mechanisms such as inhibition of protein and cell wall synthesi, and distruption of metabolic processes. Using gas chromatography- mass spectrometry (GC-MS) analysis, pyrazole and phenanthroline derivatives were detectes moss among the majorconstituents that potentially contribute to the antimeoheaded activity. The result scientifically validated the plants used in Jakun traditionaI medicine,displayed promising therapeutic properties and further studies in this direction world head to the get antimycobacterial agents
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Sabran, Siti Fatimah
author_facet	Sabran, Siti Fatimah
author_sort	Sabran, Siti Fatimah
title	Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor
title_short	Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor
title_full	Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor
title_fullStr	Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor
title_full_unstemmed	Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor
title_sort	documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in johor
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Sains, Teknologi dan Pembangunan Insan
publishDate	2016
url	http://eprints.uthm.edu.my/10042/2/24p%20SITI%20FATIMAH%20SABRAN.pdf http://eprints.uthm.edu.my/10042/1/SITI%20FATIMAH%20SABRAN%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/10042/3/SITI%20FATIMAH%20SABRAN%20WATERMARK.pdf
_version_	1783728960710901760

Documentation, antimycobacterial activity, and phytochemical profiling of selected medicinal plants used by the jakun community in Johor

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