The optimization of graphene sensing layer against Escherichia coli

In the last decade, biosensor have been developed to detect E. coli. The system is complex with a receptor detecting specific target and producing a signal transducer to be a readout data. Previous E. coli sensors lacked selectivity, that potentially could detect other bacteria. In order to rectify...

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Main Author:	Muslihati, Atqiya
Format:	Thesis
Language:	English English English
Published:	2021
Subjects:	T173.2-174.5 Technological change
Online Access:	http://eprints.uthm.edu.my/1092/1/24p%20ATQIYA%20MUSLIHATI.pdf http://eprints.uthm.edu.my/1092/2/ATQIYA%20MUSLIHATI%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/1092/3/ATQIYA%20MUSLIHATI%20WATERMARK.pdf
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spelling	my-uthm-ep.10922021-08-22T08:39:20Z The optimization of graphene sensing layer against Escherichia coli 2021-03 Muslihati, Atqiya T173.2-174.5 Technological change In the last decade, biosensor have been developed to detect E. coli. The system is complex with a receptor detecting specific target and producing a signal transducer to be a readout data. Previous E. coli sensors lacked selectivity, that potentially could detect other bacteria. In order to rectify this problem, this study aims to investigate the immobilization of anti–O and K E. coli antibodies on a SiO2/graphene film through the usage of a Pyrene butyric acid N–hydroxysuccinimide (PBANHS) linker. The investigation used some instruments like Raman spectroscopy, Atomic Force Microscopy (AFM), Field Effect Scanning Electron Microscopy (FE–SEM), and Current–Voltage (I–V) meter. In this research, the antibody was successfully immobilized on SiO2/graphene evidenced by the presence of pyrene (C–C) peak at <1000 cm–1 and (S–H) hybridization peak at 2506.25 cm–1 as PBANHS/anti–O and K E. coli antibody fingerprint in Raman spectra. Graphene height surface distribution increased 7.893 nm after PBANHS assembly and 0.364 nm after antibody immobilization. On the other hand, graphene maximum height decreased 0.46 nm after PBANHS assembly and 0.33 nm after antibody immobilization. Based on the electrical resistance, the sensing layer was able to detect E. coli against Staphylococcus aureus (S. aureus) with resistance difference 3.97 Ω and Limit of Detection (LOD) 16 CFU/mL. FE–SEM image shows the dispersion and attachment of E coli on the surface of the sensing layer, compared to the clustering of S. aureus. This new investigation lead to a new potential of specific immobilized anti–O and K E. coli antibodies on SiO2/graphene film as a selective sensing layer on E. coli sensor system. 2021-03 Thesis http://eprints.uthm.edu.my/1092/ http://eprints.uthm.edu.my/1092/1/24p%20ATQIYA%20MUSLIHATI.pdf text en public http://eprints.uthm.edu.my/1092/2/ATQIYA%20MUSLIHATI%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/1092/3/ATQIYA%20MUSLIHATI%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Faculty of Applied Science and Technology
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	T173.2-174.5 Technological change
spellingShingle	T173.2-174.5 Technological change Muslihati, Atqiya The optimization of graphene sensing layer against Escherichia coli
description	In the last decade, biosensor have been developed to detect E. coli. The system is complex with a receptor detecting specific target and producing a signal transducer to be a readout data. Previous E. coli sensors lacked selectivity, that potentially could detect other bacteria. In order to rectify this problem, this study aims to investigate the immobilization of anti–O and K E. coli antibodies on a SiO2/graphene film through the usage of a Pyrene butyric acid N–hydroxysuccinimide (PBANHS) linker. The investigation used some instruments like Raman spectroscopy, Atomic Force Microscopy (AFM), Field Effect Scanning Electron Microscopy (FE–SEM), and Current–Voltage (I–V) meter. In this research, the antibody was successfully immobilized on SiO2/graphene evidenced by the presence of pyrene (C–C) peak at <1000 cm–1 and (S–H) hybridization peak at 2506.25 cm–1 as PBANHS/anti–O and K E. coli antibody fingerprint in Raman spectra. Graphene height surface distribution increased 7.893 nm after PBANHS assembly and 0.364 nm after antibody immobilization. On the other hand, graphene maximum height decreased 0.46 nm after PBANHS assembly and 0.33 nm after antibody immobilization. Based on the electrical resistance, the sensing layer was able to detect E. coli against Staphylococcus aureus (S. aureus) with resistance difference 3.97 Ω and Limit of Detection (LOD) 16 CFU/mL. FE–SEM image shows the dispersion and attachment of E coli on the surface of the sensing layer, compared to the clustering of S. aureus. This new investigation lead to a new potential of specific immobilized anti–O and K E. coli antibodies on SiO2/graphene film as a selective sensing layer on E. coli sensor system.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Muslihati, Atqiya
author_facet	Muslihati, Atqiya
author_sort	Muslihati, Atqiya
title	The optimization of graphene sensing layer against Escherichia coli
title_short	The optimization of graphene sensing layer against Escherichia coli
title_full	The optimization of graphene sensing layer against Escherichia coli
title_fullStr	The optimization of graphene sensing layer against Escherichia coli
title_full_unstemmed	The optimization of graphene sensing layer against Escherichia coli
title_sort	optimization of graphene sensing layer against escherichia coli
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Faculty of Applied Science and Technology
publishDate	2021
url	http://eprints.uthm.edu.my/1092/1/24p%20ATQIYA%20MUSLIHATI.pdf http://eprints.uthm.edu.my/1092/2/ATQIYA%20MUSLIHATI%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/1092/3/ATQIYA%20MUSLIHATI%20WATERMARK.pdf
_version_	1747830736092659712

The optimization of graphene sensing layer against Escherichia coli

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