Tapered microfluidic device for multi-particle separation based on sedimentation principle

This thesis presents a label-free tapered microfluidic device for a passive multi-particle separation. Separation process plays a significant role in various industries for example, biomedical diagnostic, food processing and substance purification. The growing needs for continuous separation process...

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Main Author:	Ahmad, Ida Laila
Format:	Thesis
Language:	English
Published:	2017
Subjects:	TJ836-927 Hydraulic machinery
Online Access:	http://eprints.uthm.edu.my/725/1/24p%20IDA%20LAILA%20AHMAD.pdf
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record_format	uketd_dc
spelling	my-uthm-ep.7252021-08-30T07:25:23Z Tapered microfluidic device for multi-particle separation based on sedimentation principle 2017-12 Ahmad, Ida Laila TJ836-927 Hydraulic machinery This thesis presents a label-free tapered microfluidic device for a passive multi-particle separation. Separation process plays a significant role in various industries for example, biomedical diagnostic, food processing and substance purification. The growing needs for continuous separation process lead to the creation of numerous microfluidic based separation devices. Currently, microfluidic based separation devices are associated with limitations in terms of design complexity, sample purity and separation throughput. Therefore, a simple novel passive tapered microfluidic separation device with various taper angles (6°, 12°, 20° and 25°) is proposed for high purity separation of biological and non-biological samples. The device utilizes coupling mechanism between hydrodynamic separations along with sedimentation effect for enhancement of sample purity. Computer-aided design software was employed during design stage while Finite Element Analysis (FEA) software was used for device design’s optimization. The device was fabricated using a soft lithography technique and was characterized in terms of physical dimensions and leakage conditions. Size based separation simulations using FEA were carried out for 3 μm and 10 μm diameters polystyrene (PS) microbeads samples as well as a mixture of 3 μm PS microbeads and Human Cervical Epithelial Carcinoma (HeLa) cells. Through FEA simulations, larger particles were collected at Outlet 1 and small particles were collected at Outlet 2 using 20° and 25° tapered devices. Furthermore, experimental tests were conducted with similar settings and samples as in the simulations. Successful multi-particle separations were observed using 20° and 25° tapered devices at 0.5 to 3.0 μl/min flow rates. These results were in agreement with simulation results obtained. Highest purity of 98% was achieved for both samples with the use of 3.0 μl/min flow rate. As a conclusion, a passive tapered microfluidic device capable of multi-particle separation at high sample purity was developed. 2017-12 Thesis http://eprints.uthm.edu.my/725/ http://eprints.uthm.edu.my/725/1/24p%20IDA%20LAILA%20AHMAD.pdf text en public phd doctoral Universiti Teknologi Malaysia Fakulti Kejuruteraan Elektrik
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English
topic	TJ836-927 Hydraulic machinery
spellingShingle	TJ836-927 Hydraulic machinery Ahmad, Ida Laila Tapered microfluidic device for multi-particle separation based on sedimentation principle
description	This thesis presents a label-free tapered microfluidic device for a passive multi-particle separation. Separation process plays a significant role in various industries for example, biomedical diagnostic, food processing and substance purification. The growing needs for continuous separation process lead to the creation of numerous microfluidic based separation devices. Currently, microfluidic based separation devices are associated with limitations in terms of design complexity, sample purity and separation throughput. Therefore, a simple novel passive tapered microfluidic separation device with various taper angles (6°, 12°, 20° and 25°) is proposed for high purity separation of biological and non-biological samples. The device utilizes coupling mechanism between hydrodynamic separations along with sedimentation effect for enhancement of sample purity. Computer-aided design software was employed during design stage while Finite Element Analysis (FEA) software was used for device design’s optimization. The device was fabricated using a soft lithography technique and was characterized in terms of physical dimensions and leakage conditions. Size based separation simulations using FEA were carried out for 3 μm and 10 μm diameters polystyrene (PS) microbeads samples as well as a mixture of 3 μm PS microbeads and Human Cervical Epithelial Carcinoma (HeLa) cells. Through FEA simulations, larger particles were collected at Outlet 1 and small particles were collected at Outlet 2 using 20° and 25° tapered devices. Furthermore, experimental tests were conducted with similar settings and samples as in the simulations. Successful multi-particle separations were observed using 20° and 25° tapered devices at 0.5 to 3.0 μl/min flow rates. These results were in agreement with simulation results obtained. Highest purity of 98% was achieved for both samples with the use of 3.0 μl/min flow rate. As a conclusion, a passive tapered microfluidic device capable of multi-particle separation at high sample purity was developed.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ahmad, Ida Laila
author_facet	Ahmad, Ida Laila
author_sort	Ahmad, Ida Laila
title	Tapered microfluidic device for multi-particle separation based on sedimentation principle
title_short	Tapered microfluidic device for multi-particle separation based on sedimentation principle
title_full	Tapered microfluidic device for multi-particle separation based on sedimentation principle
title_fullStr	Tapered microfluidic device for multi-particle separation based on sedimentation principle
title_full_unstemmed	Tapered microfluidic device for multi-particle separation based on sedimentation principle
title_sort	tapered microfluidic device for multi-particle separation based on sedimentation principle
granting_institution	Universiti Teknologi Malaysia
granting_department	Fakulti Kejuruteraan Elektrik
publishDate	2017
url	http://eprints.uthm.edu.my/725/1/24p%20IDA%20LAILA%20AHMAD.pdf
_version_	1747830668092506112

Tapered microfluidic device for multi-particle separation based on sedimentation principle

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