Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production

Xylanase is a major hydrolysis enzyme that is important for xylan degradation in applications such as paper pulping, food additive production and animal feedstocks. It is typically found in fungi with low productivity and complex processes. As a result, an alternative method for increasing xylanase...

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Main Author:	Abdul Manaf, Shoriya Aruni
Format:	Thesis
Language:	English English English
Published:	2022
Subjects:	TP Chemical technology
Online Access:	http://eprints.uthm.edu.my/8486/1/24p%20SHORIYA%20ARUNI%20ABDUL%20MANAF.pdf http://eprints.uthm.edu.my/8486/2/SHORIYA%20ARUNI%20ABDUL%20MANAF%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/8486/3/SHORIYA%20ARUNI%20ABDUL%20MANAF%20WATERMARK.pdf
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spelling	my-uthm-ep.84862023-04-02T00:53:18Z Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production 2022-09 Abdul Manaf, Shoriya Aruni TP Chemical technology Xylanase is a major hydrolysis enzyme that is important for xylan degradation in applications such as paper pulping, food additive production and animal feedstocks. It is typically found in fungi with low productivity and complex processes. As a result, an alternative method for increasing xylanase production that is simple and less time-consuming is desired. The goal of this research is to produce a large-scale xylanase by immobilizing recombinant Kluyveromyces lactis with carbon nanomaterial and to apply a direct whole cell biocatalyst method for xylooligosaccharides production. Therefore, four carbon nanomaterials were screened using the pretreatment process that measured xylanase activity and cell growth. Carbon nanotubes (CNT) and graphene oxide (GO) were analyzed and their immobilization and culture condition factors were optimized using Response Surface Methodology (RSM) with different design models, as well as large-scale production process using a bioreactor. Analysis on the carbon nanomaterial was done using a Field Emission Scanning Electron Microscopy with Energy Dispersive X-ray (FESEM-EDX) and Fourier Transform Infrared spectroscopy (FTIR) while Ultra High-Performance Liquid Chromatography (UHPLC) was used to analyze the final sugar product. The most important factors in xylanase production with low cell leakage are cell loading and agar concentration. Following RSM screening and optimization, the xylanase production from free cells (1.39 U/mL) increased tenfold after cell immobilization (10.30 U/mL), and increased to 15 U/mL during the upscale process in the bioreactor. The immobilized cells can be reused for up to 7 fermentation cycles and stored at 4 ℃ for up to 90 days. The end products of lignocellulosic biomass bioconversion are xylobiose and xylotriose. Cell immobilization with carbon nanomaterials has been shown to successfully enhance xylanase production, opening up a new path to improved bioprocessing, particularly for the production of enzymes with reusability and long-term storage. 2022-09 Thesis http://eprints.uthm.edu.my/8486/ http://eprints.uthm.edu.my/8486/1/24p%20SHORIYA%20ARUNI%20ABDUL%20MANAF.pdf text en public http://eprints.uthm.edu.my/8486/2/SHORIYA%20ARUNI%20ABDUL%20MANAF%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/8486/3/SHORIYA%20ARUNI%20ABDUL%20MANAF%20WATERMARK.pdf text en validuser phd doctoral Universiti Tun Hussein Onn Malaysia Fakulti Sains Gunaan dan Teknologi
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Abdul Manaf, Shoriya Aruni Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
description	Xylanase is a major hydrolysis enzyme that is important for xylan degradation in applications such as paper pulping, food additive production and animal feedstocks. It is typically found in fungi with low productivity and complex processes. As a result, an alternative method for increasing xylanase production that is simple and less time-consuming is desired. The goal of this research is to produce a large-scale xylanase by immobilizing recombinant Kluyveromyces lactis with carbon nanomaterial and to apply a direct whole cell biocatalyst method for xylooligosaccharides production. Therefore, four carbon nanomaterials were screened using the pretreatment process that measured xylanase activity and cell growth. Carbon nanotubes (CNT) and graphene oxide (GO) were analyzed and their immobilization and culture condition factors were optimized using Response Surface Methodology (RSM) with different design models, as well as large-scale production process using a bioreactor. Analysis on the carbon nanomaterial was done using a Field Emission Scanning Electron Microscopy with Energy Dispersive X-ray (FESEM-EDX) and Fourier Transform Infrared spectroscopy (FTIR) while Ultra High-Performance Liquid Chromatography (UHPLC) was used to analyze the final sugar product. The most important factors in xylanase production with low cell leakage are cell loading and agar concentration. Following RSM screening and optimization, the xylanase production from free cells (1.39 U/mL) increased tenfold after cell immobilization (10.30 U/mL), and increased to 15 U/mL during the upscale process in the bioreactor. The immobilized cells can be reused for up to 7 fermentation cycles and stored at 4 ℃ for up to 90 days. The end products of lignocellulosic biomass bioconversion are xylobiose and xylotriose. Cell immobilization with carbon nanomaterials has been shown to successfully enhance xylanase production, opening up a new path to improved bioprocessing, particularly for the production of enzymes with reusability and long-term storage.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Abdul Manaf, Shoriya Aruni
author_facet	Abdul Manaf, Shoriya Aruni
author_sort	Abdul Manaf, Shoriya Aruni
title	Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_short	Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_full	Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_fullStr	Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_full_unstemmed	Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
title_sort	cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Sains Gunaan dan Teknologi
publishDate	2022
url	http://eprints.uthm.edu.my/8486/1/24p%20SHORIYA%20ARUNI%20ABDUL%20MANAF.pdf http://eprints.uthm.edu.my/8486/2/SHORIYA%20ARUNI%20ABDUL%20MANAF%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/8486/3/SHORIYA%20ARUNI%20ABDUL%20MANAF%20WATERMARK.pdf
_version_	1776103357312663552

Cell immobilization of recombinant kluyveromyces lactis on carbon nanomaterial for the improvement of xylanase production

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