Investigation on hela cells behaviour induced with pulse electric field for wound healing application

This study focuses on the investigation of pulsed electric field (PEF) exposure effect on HeLa cells (cervical cancer cells) for in-vitro experiments. The study focused mainly on real time experimental setup for cell morphological properties imaging. In the experimental setup, a modified EC magne...

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Main Author:	Milad Zaltum, Mohamed Ahmed
Format:	Thesis
Language:	English English English
Published:	2017
Subjects:	R Medicine (General)
Online Access:	http://eprints.uthm.edu.my/8504/2/24p%20MOHAMED%20AHMED%20MILDAD%20ZALTUM.pdf http://eprints.uthm.edu.my/8504/1/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/8504/3/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20WATERMARK.pdf
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spelling	my-uthm-ep.85042023-04-02T01:23:54Z Investigation on hela cells behaviour induced with pulse electric field for wound healing application 2017-04 Milad Zaltum, Mohamed Ahmed R Medicine (General) R131-687 History of medicine. Medical expeditions This study focuses on the investigation of pulsed electric field (PEF) exposure effect on HeLa cells (cervical cancer cells) for in-vitro experiments. The study focused mainly on real time experimental setup for cell morphological properties imaging. In the experimental setup, a modified EC magnetic chamber with incubator system is used to maintain the real time in-vitro environment for exposing the HeLa cells to high electric field. A Nikon inverted microscope (Ti-series) with Metamorph® time lapse application is utlized for image capturing and video recording. The first investigation is to look at the proliferation rate of HeLa cells within 72 hours inside the modified chamber. This first investigation is utmost important to see if there is any effect from the PEF exposure. From this, it was found that the HeLa cell growth rate increased up to 50% faster when applied Electroporation (EP) in comparison to the cell without EP treatment. The investigation was continued to look at the best PEF parameter that assisted in the growth rate of HeLa cells. These investegations motivates the need of finding the best parameters for PEF exposure that are suitable for HeLa cell reversible condition. This study continues to look at the relation between amplitude and duration of PEF effect on the HeLa cell growth rate. The cells were subjected to single pulse, constant field strength of l kV/cm and pulse durations ranging from 30μs to 600μs. It was found that at l00μs pulse duration the HeLa cell growth rate increased dramatically and achieving confluency faster in comparison to the cells exposed with other pulse durations. After obtaining the best parameter for HeLa cell ( l kV/cm, l00μs, & single pulse) the potential application of the EP technique for wound healing was explored. The result of the exposed cells to PEF revealed a five times faster healing rate than control group. Additionally, we have used Microcontact printing (MCP) technique for cell guidance and assembling. The results indicates that the cells aligned and elongated more on fibronectin pattern substrate under PEF than without PEF. Thus, PEF usage on biological cells would enable a novel method for assisting drug free wound repair systems and many other potential biomedical engineering applications. 2017-04 Thesis http://eprints.uthm.edu.my/8504/ http://eprints.uthm.edu.my/8504/2/24p%20MOHAMED%20AHMED%20MILDAD%20ZALTUM.pdf text en public http://eprints.uthm.edu.my/8504/1/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/8504/3/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20WATERMARK.pdf text en validuser phd doctoral Universiti Tun Hussein Onn Malaysia Fakulti Kejuruteraan Elektrik dan Elektronik
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	R Medicine (General) R Medicine (General)
spellingShingle	R Medicine (General) R Medicine (General) Milad Zaltum, Mohamed Ahmed Investigation on hela cells behaviour induced with pulse electric field for wound healing application
description	This study focuses on the investigation of pulsed electric field (PEF) exposure effect on HeLa cells (cervical cancer cells) for in-vitro experiments. The study focused mainly on real time experimental setup for cell morphological properties imaging. In the experimental setup, a modified EC magnetic chamber with incubator system is used to maintain the real time in-vitro environment for exposing the HeLa cells to high electric field. A Nikon inverted microscope (Ti-series) with Metamorph® time lapse application is utlized for image capturing and video recording. The first investigation is to look at the proliferation rate of HeLa cells within 72 hours inside the modified chamber. This first investigation is utmost important to see if there is any effect from the PEF exposure. From this, it was found that the HeLa cell growth rate increased up to 50% faster when applied Electroporation (EP) in comparison to the cell without EP treatment. The investigation was continued to look at the best PEF parameter that assisted in the growth rate of HeLa cells. These investegations motivates the need of finding the best parameters for PEF exposure that are suitable for HeLa cell reversible condition. This study continues to look at the relation between amplitude and duration of PEF effect on the HeLa cell growth rate. The cells were subjected to single pulse, constant field strength of l kV/cm and pulse durations ranging from 30μs to 600μs. It was found that at l00μs pulse duration the HeLa cell growth rate increased dramatically and achieving confluency faster in comparison to the cells exposed with other pulse durations. After obtaining the best parameter for HeLa cell ( l kV/cm, l00μs, & single pulse) the potential application of the EP technique for wound healing was explored. The result of the exposed cells to PEF revealed a five times faster healing rate than control group. Additionally, we have used Microcontact printing (MCP) technique for cell guidance and assembling. The results indicates that the cells aligned and elongated more on fibronectin pattern substrate under PEF than without PEF. Thus, PEF usage on biological cells would enable a novel method for assisting drug free wound repair systems and many other potential biomedical engineering applications.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Milad Zaltum, Mohamed Ahmed
author_facet	Milad Zaltum, Mohamed Ahmed
author_sort	Milad Zaltum, Mohamed Ahmed
title	Investigation on hela cells behaviour induced with pulse electric field for wound healing application
title_short	Investigation on hela cells behaviour induced with pulse electric field for wound healing application
title_full	Investigation on hela cells behaviour induced with pulse electric field for wound healing application
title_fullStr	Investigation on hela cells behaviour induced with pulse electric field for wound healing application
title_full_unstemmed	Investigation on hela cells behaviour induced with pulse electric field for wound healing application
title_sort	investigation on hela cells behaviour induced with pulse electric field for wound healing application
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Kejuruteraan Elektrik dan Elektronik
publishDate	2017
url	http://eprints.uthm.edu.my/8504/2/24p%20MOHAMED%20AHMED%20MILDAD%20ZALTUM.pdf http://eprints.uthm.edu.my/8504/1/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/8504/3/MOHAMED%20AHMED%20MILDAD%20ZALTUM%20WATERMARK.pdf
_version_	1776103361388478464

Investigation on hela cells behaviour induced with pulse electric field for wound healing application

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