Hardware development for MURoC rope climbing robot using sloth method

he conventional 2D culture technique used for producing mono layer of cells in the petri dish was widely practiced in the life sciences laboratory. However, this culture technique produced the organisation of cells that were far from the natural tissue and the outcomes for biological studies were no...

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Main Author:	Thong, Kok Tung
Format:	Thesis
Language:	English English English
Published:	2016
Subjects:	TJ Mechanical engineering and machinery
Online Access:	http://eprints.uthm.edu.my/9994/1/THONG%20KOK%20TUNG%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/9994/2/24p%20THONG%20KOK%20TUNG%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/9994/3/THONG%20KOK%20TUNG%20WATERMARK.pdf
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id	my-uthm-ep.9994
record_format	uketd_dc
spelling	my-uthm-ep.99942023-09-13T07:35:38Z Hardware development for MURoC rope climbing robot using sloth method 2016-01 Thong, Kok Tung TJ Mechanical engineering and machinery TJ210.2-211.47 Mechanical devices and figures. Automata. Ingenious mechanisms. Robots (General) he conventional 2D culture technique used for producing mono layer of cells in the petri dish was widely practiced in the life sciences laboratory. However, this culture technique produced the organisation of cells that were far from the natural tissue and the outcomes for biological studies were not reliable. In this research, liquid crystal was exploited as a new culture technique to produce 3D micro-tissues of human keratinocyte cell lines (HaCaT). The development of 3D micro-tissues technique initiated with the optimisation of different cell density on the coated cholesteryl ester liquid crystal (CELC) substrate to obtain a higher number and larger sized of microtissues. The investigation of 3D micro-tissues physical characteristic included the analyses of cell-to-cell interaction, cell organisation, biochemistry element in microtissues, cell responses to enzymatic dissociation treatment, immunostaining and cell viability study of micro-tissues. A microfluidic vibrational cleaner constituted polydimethylsiloxane (PDMS) microfluidic chip and electronic system were developed to remove the residue liquid crystal from the extracted micro-tissues. Based on the cell monitoring and immunostaining, HaCaT cells were found with the ability to self-aggregate with adjacent cells and organised into microspheroid on liquid crystal substrate. This culture technique was able to maintain the cell viability of the micro-tissues around 80%. In addition, the micro-tissues presented potential to be re-cultured on polystyrene culture dish again. The liquid crystal residue on the extracted micro-tissues was effectively cleaned by the vibrational cleaner at a frequency of 148 Hz and acceleration of 0.89 Gnns- The liquid crystal based 3D micro-tissue culture technique and the cleaning device was reliable to produce cleaned micro-tissues 2016-01 Thesis http://eprints.uthm.edu.my/9994/ http://eprints.uthm.edu.my/9994/1/THONG%20KOK%20TUNG%20COPYRIGHT%20DECLARATION.pdf text en public http://eprints.uthm.edu.my/9994/2/24p%20THONG%20KOK%20TUNG%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/9994/3/THONG%20KOK%20TUNG%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Fakulti Kejuruteraan Elektrik dan Elektronik
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	TJ Mechanical engineering and machinery TJ Mechanical engineering and machinery
spellingShingle	TJ Mechanical engineering and machinery TJ Mechanical engineering and machinery Thong, Kok Tung Hardware development for MURoC rope climbing robot using sloth method
description	he conventional 2D culture technique used for producing mono layer of cells in the petri dish was widely practiced in the life sciences laboratory. However, this culture technique produced the organisation of cells that were far from the natural tissue and the outcomes for biological studies were not reliable. In this research, liquid crystal was exploited as a new culture technique to produce 3D micro-tissues of human keratinocyte cell lines (HaCaT). The development of 3D micro-tissues technique initiated with the optimisation of different cell density on the coated cholesteryl ester liquid crystal (CELC) substrate to obtain a higher number and larger sized of microtissues. The investigation of 3D micro-tissues physical characteristic included the analyses of cell-to-cell interaction, cell organisation, biochemistry element in microtissues, cell responses to enzymatic dissociation treatment, immunostaining and cell viability study of micro-tissues. A microfluidic vibrational cleaner constituted polydimethylsiloxane (PDMS) microfluidic chip and electronic system were developed to remove the residue liquid crystal from the extracted micro-tissues. Based on the cell monitoring and immunostaining, HaCaT cells were found with the ability to self-aggregate with adjacent cells and organised into microspheroid on liquid crystal substrate. This culture technique was able to maintain the cell viability of the micro-tissues around 80%. In addition, the micro-tissues presented potential to be re-cultured on polystyrene culture dish again. The liquid crystal residue on the extracted micro-tissues was effectively cleaned by the vibrational cleaner at a frequency of 148 Hz and acceleration of 0.89 Gnns- The liquid crystal based 3D micro-tissue culture technique and the cleaning device was reliable to produce cleaned micro-tissues
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Thong, Kok Tung
author_facet	Thong, Kok Tung
author_sort	Thong, Kok Tung
title	Hardware development for MURoC rope climbing robot using sloth method
title_short	Hardware development for MURoC rope climbing robot using sloth method
title_full	Hardware development for MURoC rope climbing robot using sloth method
title_fullStr	Hardware development for MURoC rope climbing robot using sloth method
title_full_unstemmed	Hardware development for MURoC rope climbing robot using sloth method
title_sort	hardware development for muroc rope climbing robot using sloth method
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Kejuruteraan Elektrik dan Elektronik
publishDate	2016
url	http://eprints.uthm.edu.my/9994/1/THONG%20KOK%20TUNG%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/9994/2/24p%20THONG%20KOK%20TUNG%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/9994/3/THONG%20KOK%20TUNG%20WATERMARK.pdf
_version_	1783728982349316096

Hardware development for MURoC rope climbing robot using sloth method

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