Characterisation of mannanase from Pontibacillus sp. CL43

Lignocellulosic biomass is a renewable feedstock for biofuel production. Pretreatment is performed to destroy the biomass recalcitrant structure subsequently allow enzymes to hydrolyse cellulose and hemicellulose for sugars production. These sugars could be used for biofuel production. In chemical p...

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Main Author:	Tan, Sing Ngoh
Format:	Thesis
Language:	English
Published:	2020
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/101923/1/TanSingNgohMFS2020.pdf
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id	my-utm-ep.101923
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spelling	my-utm-ep.1019232023-07-25T09:31:16Z Characterisation of mannanase from Pontibacillus sp. CL43 2020 Tan, Sing Ngoh QD Chemistry Lignocellulosic biomass is a renewable feedstock for biofuel production. Pretreatment is performed to destroy the biomass recalcitrant structure subsequently allow enzymes to hydrolyse cellulose and hemicellulose for sugars production. These sugars could be used for biofuel production. In chemical pretreatment of lignocellulosic biomass, metal salts (KCl, CaCl2 and MgCl2) and surfactants (Tween 20, Tween 80 and Triton X-100) are used. These compounds retain in the biomass after the treatment and affect the performance of enzymes in following hydrolysis process. Hence, enzymes with metal- and surfactant-tolerance characteristics are preferable in biofuel production. This project focused on characterising Pontibacillus sp. CL43 and its mannanase which is responsible for degradation of mannan, a component present in lignocellulosic biomass. Strain CL43 is a Gram positive bacterium with rod-shaped, endospore-forming, catalase- and oxidase-positive. Strain CL43 could hydrolyse bile esculin, casein, starch, xylan, Tween 20, Tween 40, Tween 60 and Tween 80. Strain CL43 was susceptible to ampicillin, carbenicillin, doxycycline, gentamicin, minocycline, neomycin, oxacillin, penicillin G, piperacillin, polymyxin B, rifampicin, streptomycin and tetracycline. Mannanase of strain CL43 exhibited optimal activity at 60 °C, pH 7 and 2% (w/v) NaCl. Mannanase activity was found to be stable in the presence of metal ions (Ca2+, Cd2+, Mn2+, K+, Mg2+ and Co2+ with relative activity ranged from 87-128%) and surfactants (Tween 20, Tween 40, Tween 60, Tween 80 and Triton X-100 with relative activity ranged from 88-107%). Mannanase of strain CL43 with good tolerance to these metal ions and surfactants indicated it is a good candidate to be used for lignocellulosic biomass hydrolysis. 2020 Thesis http://eprints.utm.my/id/eprint/101923/ http://eprints.utm.my/id/eprint/101923/1/TanSingNgohMFS2020.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146356 masters Universiti Teknologi Malaysia Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Tan, Sing Ngoh Characterisation of mannanase from Pontibacillus sp. CL43
description	Lignocellulosic biomass is a renewable feedstock for biofuel production. Pretreatment is performed to destroy the biomass recalcitrant structure subsequently allow enzymes to hydrolyse cellulose and hemicellulose for sugars production. These sugars could be used for biofuel production. In chemical pretreatment of lignocellulosic biomass, metal salts (KCl, CaCl2 and MgCl2) and surfactants (Tween 20, Tween 80 and Triton X-100) are used. These compounds retain in the biomass after the treatment and affect the performance of enzymes in following hydrolysis process. Hence, enzymes with metal- and surfactant-tolerance characteristics are preferable in biofuel production. This project focused on characterising Pontibacillus sp. CL43 and its mannanase which is responsible for degradation of mannan, a component present in lignocellulosic biomass. Strain CL43 is a Gram positive bacterium with rod-shaped, endospore-forming, catalase- and oxidase-positive. Strain CL43 could hydrolyse bile esculin, casein, starch, xylan, Tween 20, Tween 40, Tween 60 and Tween 80. Strain CL43 was susceptible to ampicillin, carbenicillin, doxycycline, gentamicin, minocycline, neomycin, oxacillin, penicillin G, piperacillin, polymyxin B, rifampicin, streptomycin and tetracycline. Mannanase of strain CL43 exhibited optimal activity at 60 °C, pH 7 and 2% (w/v) NaCl. Mannanase activity was found to be stable in the presence of metal ions (Ca2+, Cd2+, Mn2+, K+, Mg2+ and Co2+ with relative activity ranged from 87-128%) and surfactants (Tween 20, Tween 40, Tween 60, Tween 80 and Triton X-100 with relative activity ranged from 88-107%). Mannanase of strain CL43 with good tolerance to these metal ions and surfactants indicated it is a good candidate to be used for lignocellulosic biomass hydrolysis.
format	Thesis
qualification_level	Master's degree
author	Tan, Sing Ngoh
author_facet	Tan, Sing Ngoh
author_sort	Tan, Sing Ngoh
title	Characterisation of mannanase from Pontibacillus sp. CL43
title_short	Characterisation of mannanase from Pontibacillus sp. CL43
title_full	Characterisation of mannanase from Pontibacillus sp. CL43
title_fullStr	Characterisation of mannanase from Pontibacillus sp. CL43
title_full_unstemmed	Characterisation of mannanase from Pontibacillus sp. CL43
title_sort	characterisation of mannanase from pontibacillus sp. cl43
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Science
publishDate	2020
url	http://eprints.utm.my/id/eprint/101923/1/TanSingNgohMFS2020.pdf
_version_	1776100804170612736

Characterisation of mannanase from Pontibacillus sp. CL43

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