Production of biosurfactant by locally isolated bacteria from petrochemical waste

Ten bacterial strains previously isolated from petrochemical wastes were selected for the screening of biosurfactant producer(s), via four different methods; (i) surface tension measurements, (ii) blood hemolysis test, (iii) drop-collapsing test, and (iv) bacterial adherence to hydrocarbon (BATH) te...

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Main Author:	Mohd. Zawawi, Rusniza
Format:	Thesis
Language:	English
Published:	2005
Subjects:	Q Science (General) QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/3216/1/RuznizaMohdZawawiMFS2005.pdf
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spelling	my-utm-ep.32162018-06-26T07:56:23Z Production of biosurfactant by locally isolated bacteria from petrochemical waste 2005-12 Mohd. Zawawi, Rusniza Q Science (General) QD Chemistry Ten bacterial strains previously isolated from petrochemical wastes were selected for the screening of biosurfactant producer(s), via four different methods; (i) surface tension measurements, (ii) blood hemolysis test, (iii) drop-collapsing test, and (iv) bacterial adherence to hydrocarbon (BATH) test. Two isolates coded AB-Cr1 and ETL-Cr1 identified as Actinobacillus sp. and Aeromonas sp., respectively were chosen to be the best candidates for biosurfactant production. Biosurfactant productions by both isolates were found to be growth-associated in all conditions tested. Biosurfactant production in glucose/crude oil medium (7.18-8.26 g/L) was found similar to that observed in crude oil-free medium (6.33-8.76 g/L). The production of biosurfactant was also studied in a fermentor using isolate AB-Cr1, as a factor of temperature, initial glucose concentration, pH and initial nitrogen concentration. The highest production of 12.45 g/L was obtained with AB-Cr1 grown in medium (pH 7) supplemented with 25mM NH4NO3 as nitrogen source and 3mM glucose as carbon source, incubated at 37Â°C under non-pH controlled strategy. TLC and FTIR characterization of crude biosurfactant produced by both isolates in medium supplemented or not with crude oil indicated the presence of lipoprotein and non-aromatic glycolipid types of biosurfactant. GC-MS analysis of fatty acid metyl esters indicated the presence of pentadecanoic acid in crude biosurfactant from both isolates as well as octadecanoic and heptadecanoic acid in the biosurfactant produced by AB-Cr1 and ETL-Cr1, respectively. The CMC of the biosurfactant produced in the presence and absence of crude oil were approximately (g/L) 1.0 and 0.1 for ABCr1, and 1.2 and 0.2 for ETL-Cr1, respectively. The biosurfactants were found capable of producing a relatively stable emulsion with hydrocarbon at pH 10. It was also found stable at various pHs (3.0-13.0 and 5.0-9.0) for AB-Cr1 and ETL-Cr1, respectively and thermostable for 1 hour at 100Â°C, based on the value of surface tension 2005-12 Thesis http://eprints.utm.my/id/eprint/3216/ http://eprints.utm.my/id/eprint/3216/1/RuznizaMohdZawawiMFS2005.pdf application/pdf en public https://www.researchgate.net/publication/48268061_Production_of_biosurfactant_by_locally_isolated_bacteria_from_petrochemical_waste masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General) QD Chemistry
spellingShingle	Q Science (General) QD Chemistry Mohd. Zawawi, Rusniza Production of biosurfactant by locally isolated bacteria from petrochemical waste
description	Ten bacterial strains previously isolated from petrochemical wastes were selected for the screening of biosurfactant producer(s), via four different methods; (i) surface tension measurements, (ii) blood hemolysis test, (iii) drop-collapsing test, and (iv) bacterial adherence to hydrocarbon (BATH) test. Two isolates coded AB-Cr1 and ETL-Cr1 identified as Actinobacillus sp. and Aeromonas sp., respectively were chosen to be the best candidates for biosurfactant production. Biosurfactant productions by both isolates were found to be growth-associated in all conditions tested. Biosurfactant production in glucose/crude oil medium (7.18-8.26 g/L) was found similar to that observed in crude oil-free medium (6.33-8.76 g/L). The production of biosurfactant was also studied in a fermentor using isolate AB-Cr1, as a factor of temperature, initial glucose concentration, pH and initial nitrogen concentration. The highest production of 12.45 g/L was obtained with AB-Cr1 grown in medium (pH 7) supplemented with 25mM NH4NO3 as nitrogen source and 3mM glucose as carbon source, incubated at 37Â°C under non-pH controlled strategy. TLC and FTIR characterization of crude biosurfactant produced by both isolates in medium supplemented or not with crude oil indicated the presence of lipoprotein and non-aromatic glycolipid types of biosurfactant. GC-MS analysis of fatty acid metyl esters indicated the presence of pentadecanoic acid in crude biosurfactant from both isolates as well as octadecanoic and heptadecanoic acid in the biosurfactant produced by AB-Cr1 and ETL-Cr1, respectively. The CMC of the biosurfactant produced in the presence and absence of crude oil were approximately (g/L) 1.0 and 0.1 for ABCr1, and 1.2 and 0.2 for ETL-Cr1, respectively. The biosurfactants were found capable of producing a relatively stable emulsion with hydrocarbon at pH 10. It was also found stable at various pHs (3.0-13.0 and 5.0-9.0) for AB-Cr1 and ETL-Cr1, respectively and thermostable for 1 hour at 100Â°C, based on the value of surface tension
format	Thesis
qualification_level	Master's degree
author	Mohd. Zawawi, Rusniza
author_facet	Mohd. Zawawi, Rusniza
author_sort	Mohd. Zawawi, Rusniza
title	Production of biosurfactant by locally isolated bacteria from petrochemical waste
title_short	Production of biosurfactant by locally isolated bacteria from petrochemical waste
title_full	Production of biosurfactant by locally isolated bacteria from petrochemical waste
title_fullStr	Production of biosurfactant by locally isolated bacteria from petrochemical waste
title_full_unstemmed	Production of biosurfactant by locally isolated bacteria from petrochemical waste
title_sort	production of biosurfactant by locally isolated bacteria from petrochemical waste
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2005
url	http://eprints.utm.my/id/eprint/3216/1/RuznizaMohdZawawiMFS2005.pdf
_version_	1747814442647683072

Production of biosurfactant by locally isolated bacteria from petrochemical waste

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