Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment

Microbial dehalogenases are involved in the biodegradation of many types of halogenated compounds. The presence of halogenated compounds in water does not only suppress the immune system of fish but adversely induces serious morbidity and mortality among cultured stocks. In this study, we attempted...

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Main Author:	Rosland Abel, Stasha Eleanor
Format:	Thesis
Language:	English
Published:	2012
Subjects:	QR Microbiology
Online Access:	http://eprints.utm.my/id/eprint/33365/1/StashaEleanorMFBB2012.pdf
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spelling	my-utm-ep.333652017-09-21T06:02:01Z Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment 2012-11 Rosland Abel, Stasha Eleanor QR Microbiology Microbial dehalogenases are involved in the biodegradation of many types of halogenated compounds. The presence of halogenated compounds in water does not only suppress the immune system of fish but adversely induces serious morbidity and mortality among cultured stocks. In this study, we attempted to screen the gut of pond-reared rohu (Labeo rohita) for isolating dehalogenase gene bacteria using molecular technique and tested the degradation ability in vitro. The present study shows eight bacterial strains studied were identified as Enterobacter mori (MK121001), Enterobacter cloacae (MK121003), Enterobacter cloacae (MK121004), Enterobacter cloacae (MK121010), Ralstonia solanacearum (121002), Acinetobacter baumannii (MK121007), Chromobacterium violaceum (MK121009) and Pantoea vagans (121011). Further analysis found three bacterial strains (MK121002, MK121007 and MK121009) were capable of degrading 2,2- dichloropropionic acid (2,2-DCP) as the sole carbon source up to a final substrate concentration of 20 mM. Their mean growth doubling time ranging from 6-23 h with the maximum of chloride ion released of 85%. Another bacterium was isolated from soil samples collected from lake water at Universiti Teknologi Malaysia, Skudai also capable of degrading 2,2-DCP. Phylogenetic analysis indicated that Serratia marcescens SE1 strain clearly shared 97% homology to the genus of Serratia marcescens according to bioinformatics analysis. Serratia marcescens has the ability to degrade 2,2-DCP with cells doubling time of 5 h and maximum chloride ion released of 38 µmolCl-/mL in the liquid growth medium. 2012-11 Thesis http://eprints.utm.my/id/eprint/33365/ http://eprints.utm.my/id/eprint/33365/1/StashaEleanorMFBB2012.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:70735?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Rosland Abel, Stasha Eleanor Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment
description	Microbial dehalogenases are involved in the biodegradation of many types of halogenated compounds. The presence of halogenated compounds in water does not only suppress the immune system of fish but adversely induces serious morbidity and mortality among cultured stocks. In this study, we attempted to screen the gut of pond-reared rohu (Labeo rohita) for isolating dehalogenase gene bacteria using molecular technique and tested the degradation ability in vitro. The present study shows eight bacterial strains studied were identified as Enterobacter mori (MK121001), Enterobacter cloacae (MK121003), Enterobacter cloacae (MK121004), Enterobacter cloacae (MK121010), Ralstonia solanacearum (121002), Acinetobacter baumannii (MK121007), Chromobacterium violaceum (MK121009) and Pantoea vagans (121011). Further analysis found three bacterial strains (MK121002, MK121007 and MK121009) were capable of degrading 2,2- dichloropropionic acid (2,2-DCP) as the sole carbon source up to a final substrate concentration of 20 mM. Their mean growth doubling time ranging from 6-23 h with the maximum of chloride ion released of 85%. Another bacterium was isolated from soil samples collected from lake water at Universiti Teknologi Malaysia, Skudai also capable of degrading 2,2-DCP. Phylogenetic analysis indicated that Serratia marcescens SE1 strain clearly shared 97% homology to the genus of Serratia marcescens according to bioinformatics analysis. Serratia marcescens has the ability to degrade 2,2-DCP with cells doubling time of 5 h and maximum chloride ion released of 38 µmolCl-/mL in the liquid growth medium.
format	Thesis
qualification_level	Master's degree
author	Rosland Abel, Stasha Eleanor
author_facet	Rosland Abel, Stasha Eleanor
author_sort	Rosland Abel, Stasha Eleanor
title	Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment
title_short	Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment
title_full	Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment
title_fullStr	Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment
title_full_unstemmed	Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment
title_sort	isolation, identification and characterization of dehalogenase producing bacteria isolated from labeo rohita and its environment
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2012
url	http://eprints.utm.my/id/eprint/33365/1/StashaEleanorMFBB2012.pdf
_version_	1747816143755673600

Isolation, identification and characterization of dehalogenase producing bacteria isolated from Labeo Rohita and its environment

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