Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum

The red microalgae Porphyridium cruentum produce many valuable compounds such as extracellular polysaccharides (EPS) that are extensively used in the industry. In this study modification of media formulation for optimal production of EPS from Porphyridium cruentum was carried out by varying nitrate,...

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Main Author:	Yaakop, Amira Suriaty
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QP Physiology
Online Access:	http://eprints.utm.my/id/eprint/35871/1/AmiraSuriatyYaakobMFBB2013.pdf
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id	my-utm-ep.35871
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spelling	my-utm-ep.358712017-09-18T07:28:20Z Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum 2013-01 Yaakop, Amira Suriaty QP Physiology The red microalgae Porphyridium cruentum produce many valuable compounds such as extracellular polysaccharides (EPS) that are extensively used in the industry. In this study modification of media formulation for optimal production of EPS from Porphyridium cruentum was carried out by varying nitrate, sulfate and glucose concentrations in the Jones 1962 media. Different concentration of each nutrient was used in the media formulation. For nitrate, the highest concentration provided in the medium at 50 mM showed the highest growth rate and EPS production, but by inhibiting the nitrate concentration, the growth is reduced while the production of EPS is increased as when a high concentration of nitrate was provided to the medium. For sulfate, increasing concentration in the media showed great decrease in growth rate, but increasing EPS production, the highest production of EPS was at 50 mM which was the highest concentration of sulfate provided in the media modification. Addition of glucose in the medium on the other hand substantially increased the growth rate and the biomass production but as the glucose concentration kept on increasing, it also inhibited the production of EPS by the algae. The growth rate of the algae did not correlate with the EPS production. The result showed that high concentration of glucose and low concentration of nitrate and sulfate inhibited the production of EPS by the microalgae and in order to increase the EPS production, medium without nitrate or a very high concentration of nitrate or and sulfate must be provided. Therefore, the optimum medium formulation for optimal growth and EPS production by Porphyridium cruentum is at either 20 mM of nitrate or without nitrate at all, 50 mM of sulfate and 0.5% (w/v) of glucose. The compositions of the EPS were also confirmed by using HPLC that showed the composition of glucose, galactose and xylose of the EPS. 2013-01 Thesis http://eprints.utm.my/id/eprint/35871/ http://eprints.utm.my/id/eprint/35871/1/AmiraSuriatyYaakobMFBB2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:70727?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QP Physiology
spellingShingle	QP Physiology Yaakop, Amira Suriaty Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum
description	The red microalgae Porphyridium cruentum produce many valuable compounds such as extracellular polysaccharides (EPS) that are extensively used in the industry. In this study modification of media formulation for optimal production of EPS from Porphyridium cruentum was carried out by varying nitrate, sulfate and glucose concentrations in the Jones 1962 media. Different concentration of each nutrient was used in the media formulation. For nitrate, the highest concentration provided in the medium at 50 mM showed the highest growth rate and EPS production, but by inhibiting the nitrate concentration, the growth is reduced while the production of EPS is increased as when a high concentration of nitrate was provided to the medium. For sulfate, increasing concentration in the media showed great decrease in growth rate, but increasing EPS production, the highest production of EPS was at 50 mM which was the highest concentration of sulfate provided in the media modification. Addition of glucose in the medium on the other hand substantially increased the growth rate and the biomass production but as the glucose concentration kept on increasing, it also inhibited the production of EPS by the algae. The growth rate of the algae did not correlate with the EPS production. The result showed that high concentration of glucose and low concentration of nitrate and sulfate inhibited the production of EPS by the microalgae and in order to increase the EPS production, medium without nitrate or a very high concentration of nitrate or and sulfate must be provided. Therefore, the optimum medium formulation for optimal growth and EPS production by Porphyridium cruentum is at either 20 mM of nitrate or without nitrate at all, 50 mM of sulfate and 0.5% (w/v) of glucose. The compositions of the EPS were also confirmed by using HPLC that showed the composition of glucose, galactose and xylose of the EPS.
format	Thesis
qualification_level	Master's degree
author	Yaakop, Amira Suriaty
author_facet	Yaakop, Amira Suriaty
author_sort	Yaakop, Amira Suriaty
title	Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum
title_short	Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum
title_full	Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum
title_fullStr	Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum
title_full_unstemmed	Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum
title_sort	modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/35871/1/AmiraSuriatyYaakobMFBB2013.pdf
_version_	1747816376149475328

Modification of media formulation for enhancing production of extracellular polysaccharides by porphyridium cruentum

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