Biotransformation of artonin E by locally isolated microorganisms

Biotransformation is a process that involves modification of the structure of a chemical compound by microbial activities. It has advantages over chemical synthesis as it is able to be operated at near room temperature, with non-extreme pH as well as it is a highly stereospecific reaction. The purpo...

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Main Author:	Lai, Yew Seng
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QP Physiology
Online Access:	http://eprints.utm.my/id/eprint/38185/1/LaiYewSengMFBSK2013.pdf
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spelling	my-utm-ep.381852018-04-12T05:42:04Z Biotransformation of artonin E by locally isolated microorganisms 2013-03 Lai, Yew Seng QP Physiology Biotransformation is a process that involves modification of the structure of a chemical compound by microbial activities. It has advantages over chemical synthesis as it is able to be operated at near room temperature, with non-extreme pH as well as it is a highly stereospecific reaction. The purpose of this research are to study the ability of selected microbes to transform artonin E, a flavonoid extracted from the bark of local plant, Artocarpus tesymanii and identify potential transformation products. 17 types of microbes were isolated from various sources, however 3 types of bacteria and 2 types of fungi were able to grow in the presence of artonin E. They were identified by 16S rRNA and Internal Transcrib Spacer Region for bacteria and fungi respectively. These microbes were Bacillus cereus, Bacillus subtilis, Bacillus pumillus, Aspergillus fumigatus and Aspergillus aculeatus. The transformation products were analysed by High Performance Liquid Chromatography (HPLC) and Thin Layer Chromatography (TLC). There was a significant peak which corresponded to the biotransformation product as shown in the HPLC chromatogram at retention time of approximately 3.9 minutes. The percentage area of the peak was shown to increase from 6.89% at day 0 to 11.80% at day 1 followed by decreasing of the percentage area of the remaining artonin E concentration in the reaction medium. This was further confirmed by TLC where a compound termed as P1 (transformation product) emerged at the Rf value of 0.37. Artonin E showed a moderate antioxidant property with IC50 value of 81.61µg/mL while the biotransformation product showed very weak antioxidant property with IC50 more than 200 µg/mL. Sample was sent for structure elucidation using Nuclear Magnetic Resonance Spectroscopy (NMR) but due to limited concentration of sample produced in the transformation, the structure could not be fully analyzed. 2013-03 Thesis http://eprints.utm.my/id/eprint/38185/ http://eprints.utm.my/id/eprint/38185/1/LaiYewSengMFBSK2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering Faculty of Bioscience and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QP Physiology
spellingShingle	QP Physiology Lai, Yew Seng Biotransformation of artonin E by locally isolated microorganisms
description	Biotransformation is a process that involves modification of the structure of a chemical compound by microbial activities. It has advantages over chemical synthesis as it is able to be operated at near room temperature, with non-extreme pH as well as it is a highly stereospecific reaction. The purpose of this research are to study the ability of selected microbes to transform artonin E, a flavonoid extracted from the bark of local plant, Artocarpus tesymanii and identify potential transformation products. 17 types of microbes were isolated from various sources, however 3 types of bacteria and 2 types of fungi were able to grow in the presence of artonin E. They were identified by 16S rRNA and Internal Transcrib Spacer Region for bacteria and fungi respectively. These microbes were Bacillus cereus, Bacillus subtilis, Bacillus pumillus, Aspergillus fumigatus and Aspergillus aculeatus. The transformation products were analysed by High Performance Liquid Chromatography (HPLC) and Thin Layer Chromatography (TLC). There was a significant peak which corresponded to the biotransformation product as shown in the HPLC chromatogram at retention time of approximately 3.9 minutes. The percentage area of the peak was shown to increase from 6.89% at day 0 to 11.80% at day 1 followed by decreasing of the percentage area of the remaining artonin E concentration in the reaction medium. This was further confirmed by TLC where a compound termed as P1 (transformation product) emerged at the Rf value of 0.37. Artonin E showed a moderate antioxidant property with IC50 value of 81.61µg/mL while the biotransformation product showed very weak antioxidant property with IC50 more than 200 µg/mL. Sample was sent for structure elucidation using Nuclear Magnetic Resonance Spectroscopy (NMR) but due to limited concentration of sample produced in the transformation, the structure could not be fully analyzed.
format	Thesis
qualification_level	Master's degree
author	Lai, Yew Seng
author_facet	Lai, Yew Seng
author_sort	Lai, Yew Seng
title	Biotransformation of artonin E by locally isolated microorganisms
title_short	Biotransformation of artonin E by locally isolated microorganisms
title_full	Biotransformation of artonin E by locally isolated microorganisms
title_fullStr	Biotransformation of artonin E by locally isolated microorganisms
title_full_unstemmed	Biotransformation of artonin E by locally isolated microorganisms
title_sort	biotransformation of artonin e by locally isolated microorganisms
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering
granting_department	Faculty of Bioscience and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/38185/1/LaiYewSengMFBSK2013.pdf
_version_	1747816525286342656

Biotransformation of artonin E by locally isolated microorganisms

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