Isolation and characterization of hydrogen producing marine photosynthetic bacteria

Photosynthetic bacteria are widely distributed in nature and are a good source of sustainable products. The aim of this work is to investigate the potential of photosynthetic bacteria from marine environments to produce hydrogen, a renewable, clean and efficient fuel. Marine environments have been i...

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Main Author:	Idris, Abdulrahman
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/48026/25/AbdulrahmanIdrisMFBME2014.pdf
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id	my-utm-ep.48026
record_format	uketd_dc
spelling	my-utm-ep.480262017-07-28T06:39:34Z Isolation and characterization of hydrogen producing marine photosynthetic bacteria 2014-07 Idris, Abdulrahman Q Science (General) Photosynthetic bacteria are widely distributed in nature and are a good source of sustainable products. The aim of this work is to investigate the potential of photosynthetic bacteria from marine environments to produce hydrogen, a renewable, clean and efficient fuel. Marine environments have been identified as major source of new natural products. Samples of water were collected from marine environments around Kuala Terengganu. Using aseptic techniques, the samples were inoculated in succinate medium and incubated under light at a temperature of 28-30oC. Two suspected photosynthetic bacteria were isolated from the sample. Both are Gram negative rods and produce carotenoid and bacteriochlorophyl photosynthetic pigments. The isolated bacteria were then investigated for their potential to produce hydrogen using acetate and synthetic waste water media containing starch as carbon sources incubated under light. The gas produced were collected and analyzed using a Residual Gas Analyzer (RGA). Both isolates show potential to produce hydrogen gas. The bacteria were then subjected to molecular characterization using 16s rRNA method to identify the isolated organisms. The two isolates were identified using NCBI BLAST tool Pseudomonas species ID1 with accession number KJ950496 and Aeromonas species ID2 with accession number KJ950497. 2014-07 Thesis http://eprints.utm.my/id/eprint/48026/ http://eprints.utm.my/id/eprint/48026/25/AbdulrahmanIdrisMFBME2014.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Idris, Abdulrahman Isolation and characterization of hydrogen producing marine photosynthetic bacteria
description	Photosynthetic bacteria are widely distributed in nature and are a good source of sustainable products. The aim of this work is to investigate the potential of photosynthetic bacteria from marine environments to produce hydrogen, a renewable, clean and efficient fuel. Marine environments have been identified as major source of new natural products. Samples of water were collected from marine environments around Kuala Terengganu. Using aseptic techniques, the samples were inoculated in succinate medium and incubated under light at a temperature of 28-30oC. Two suspected photosynthetic bacteria were isolated from the sample. Both are Gram negative rods and produce carotenoid and bacteriochlorophyl photosynthetic pigments. The isolated bacteria were then investigated for their potential to produce hydrogen using acetate and synthetic waste water media containing starch as carbon sources incubated under light. The gas produced were collected and analyzed using a Residual Gas Analyzer (RGA). Both isolates show potential to produce hydrogen gas. The bacteria were then subjected to molecular characterization using 16s rRNA method to identify the isolated organisms. The two isolates were identified using NCBI BLAST tool Pseudomonas species ID1 with accession number KJ950496 and Aeromonas species ID2 with accession number KJ950497.
format	Thesis
qualification_level	Master's degree
author	Idris, Abdulrahman
author_facet	Idris, Abdulrahman
author_sort	Idris, Abdulrahman
title	Isolation and characterization of hydrogen producing marine photosynthetic bacteria
title_short	Isolation and characterization of hydrogen producing marine photosynthetic bacteria
title_full	Isolation and characterization of hydrogen producing marine photosynthetic bacteria
title_fullStr	Isolation and characterization of hydrogen producing marine photosynthetic bacteria
title_full_unstemmed	Isolation and characterization of hydrogen producing marine photosynthetic bacteria
title_sort	isolation and characterization of hydrogen producing marine photosynthetic bacteria
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2014
url	http://eprints.utm.my/id/eprint/48026/25/AbdulrahmanIdrisMFBME2014.pdf
_version_	1747817289744384000

Isolation and characterization of hydrogen producing marine photosynthetic bacteria

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