Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics

Single cell mechanics is a vital part of single cell analysis. It has attracted great interest among scientists as cell mechanics can be linked to early diagnosis of diseases. To date, several great findings have been achieved in the study of single cell mechanics. Nevertheless, more work are requir...

Full description

Saved in:

Bibliographic Details
Main Author:	Rahman, Md. Habibur
Format:	Thesis
Language:	English
Published:	2015
Subjects:	T Technology (General)
Online Access:	http://eprints.utm.my/id/eprint/48790/25/MdHabiburRahmanMFKE2015.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.48790
record_format	uketd_dc
spelling	my-utm-ep.487902020-06-24T00:59:56Z Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics 2015-03 Rahman, Md. Habibur T Technology (General) Single cell mechanics is a vital part of single cell analysis. It has attracted great interest among scientists as cell mechanics can be linked to early diagnosis of diseases. To date, several great findings have been achieved in the study of single cell mechanics. Nevertheless, more work are required to enable the technology to be pushed to the frontier of single cell mechanics. Considering this objective, this work focuses on the technological development of two major parameters of single cell mechanics: Single Cell Wall (SCW) cutting operations (Phase 01) and Single Cell Mass (SCM) measurement (Phase 02). A saccharomyces cerevisiae yeast cell was used as a sample cell. In phase 01, a vibrating nanoneedle (tungsten) integrated with lead zirconate titanate piezoelectric actuator was used for SCW cutting operation. Two different frequencies of vibrating nanoneedle were used for cell wall cutting operation: 1 Hz and 10 Hz. For a constant penetration depth of 1.2 µm, the obtained cell nanoneedle’s velocities were 7 µm/s and 24 µm/s. Results show that faster nanoneedle causes less damage to the cell surface. In phase 02, a Lab-On-Chip microfluidics system was used for SCM measurement. SCM result was extracted from the relation between drag force applied on cell and Newton’s law of motion. Drag force on the cell has been generated by a pressure driven syringe micropump. This approach of measuring SCM was calibrated using a known mass (73.5 pico gram) of polystyrene particle of 5.2 µm diameter. Different sizes (2-7 µm diameter) of yeast cells were cultured in our laboratory. Mass of 4.4 µm diameter of yeast cell was measured as 2.12 pg. In addition, results show that single yeast cell mass increases exponentially with the increase of cell size. It is envisaged that this work i.e. combination of single cell cutting operation and single cell mass measurement system will add a significant contribution to the knowledge of cell mechanics and single cell analysis. 2015-03 Thesis http://eprints.utm.my/id/eprint/48790/ http://eprints.utm.my/id/eprint/48790/25/MdHabiburRahmanMFKE2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:87883 masters Universiti Teknologi Malaysia, Faculty of Electrical Engineering Faculty of Electrical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Rahman, Md. Habibur Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics
description	Single cell mechanics is a vital part of single cell analysis. It has attracted great interest among scientists as cell mechanics can be linked to early diagnosis of diseases. To date, several great findings have been achieved in the study of single cell mechanics. Nevertheless, more work are required to enable the technology to be pushed to the frontier of single cell mechanics. Considering this objective, this work focuses on the technological development of two major parameters of single cell mechanics: Single Cell Wall (SCW) cutting operations (Phase 01) and Single Cell Mass (SCM) measurement (Phase 02). A saccharomyces cerevisiae yeast cell was used as a sample cell. In phase 01, a vibrating nanoneedle (tungsten) integrated with lead zirconate titanate piezoelectric actuator was used for SCW cutting operation. Two different frequencies of vibrating nanoneedle were used for cell wall cutting operation: 1 Hz and 10 Hz. For a constant penetration depth of 1.2 µm, the obtained cell nanoneedle’s velocities were 7 µm/s and 24 µm/s. Results show that faster nanoneedle causes less damage to the cell surface. In phase 02, a Lab-On-Chip microfluidics system was used for SCM measurement. SCM result was extracted from the relation between drag force applied on cell and Newton’s law of motion. Drag force on the cell has been generated by a pressure driven syringe micropump. This approach of measuring SCM was calibrated using a known mass (73.5 pico gram) of polystyrene particle of 5.2 µm diameter. Different sizes (2-7 µm diameter) of yeast cells were cultured in our laboratory. Mass of 4.4 µm diameter of yeast cell was measured as 2.12 pg. In addition, results show that single yeast cell mass increases exponentially with the increase of cell size. It is envisaged that this work i.e. combination of single cell cutting operation and single cell mass measurement system will add a significant contribution to the knowledge of cell mechanics and single cell analysis.
format	Thesis
qualification_level	Master's degree
author	Rahman, Md. Habibur
author_facet	Rahman, Md. Habibur
author_sort	Rahman, Md. Habibur
title	Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics
title_short	Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics
title_full	Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics
title_fullStr	Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics
title_full_unstemmed	Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics
title_sort	vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics
granting_institution	Universiti Teknologi Malaysia, Faculty of Electrical Engineering
granting_department	Faculty of Electrical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/48790/25/MdHabiburRahmanMFKE2015.pdf
_version_	1747817472547880960

Vibrating nanoneedle and lab-on-chip microfluidics system for single cell mechanics

Similar Items