Identification and characterization of bacteria from the skin of jackfruit

Fresh-cut fruits industry is developing extensively in this global era due to busy lifestyle. However, the fresh-cut processing could be contaminated by microorganisms; resulting in food poisoning outbreaks. This study presented about the characterization and inhibition of bacteria isolated from the...

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Main Author:	Selvaratnam, Chitra
Format:	Thesis
Language:	English
Published:	2014
Subjects:	R Medicine (General)
Online Access:	http://eprints.utm.my/id/eprint/48793/25/ChitraSelvaratnamMFS2014.pdf
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spelling	my-utm-ep.487932020-06-24T01:34:08Z Identification and characterization of bacteria from the skin of jackfruit 2014-06 Selvaratnam, Chitra R Medicine (General) Fresh-cut fruits industry is developing extensively in this global era due to busy lifestyle. However, the fresh-cut processing could be contaminated by microorganisms; resulting in food poisoning outbreaks. This study presented about the characterization and inhibition of bacteria isolated from the fresh skin of jackfruit. The isolation of single colonies was done by spread plate and streak plate method. The isolates were identified by 16S rRNA analysis. Gram staining, growth profiling and 15 biochemical tests were carried out to characterize each isolates.Lastly, the isolates were treated with various antimicrobial agents by disc agar diffusion technique and the fresh skin of jackfruit was treated with antimicrobialagent, XY-12 with different conditions by spread plate technique to study the effects of antimicrobial agents on microbial growth. Experimental results demonstrated that four types of bacterial single colonies were isolated with different morphology and designated as CE1, CE2, CE3 and CE4. The 16S rRNA analysis showed that the isolates of CE1 and CE4 were Bacillus sp. strain CY-b33. Isolates of CE2 and CE3were identified as Bacillus pumilus strain SBTBP-008 and Bacillus thuringiensis strain EA26.1 respectively. All the isolates were Gram positive and in rod shaped.The growth kinetics of CE3 was highest (0.2280 h-1) compared to CE1 (0.1317 h-1)which was the lowest. Bacteria CE1 and CE2 have the highest sensitivity againstXY-12 (0.6 mL/L) and Kanamycin Sulfate respectively. Bacteria CE3 and CE4 have the highest sensitivity against Ampicillin Trihydrate and Tetracycline Hydrochloride respectively. The fresh skin of jackfruit treated with XY-12 (0.6 mL/L) and incubated at 4°C revealed high efficiency in microbial reduction at day 2 (100%) and 4 (99.72%). 2014-06 Thesis http://eprints.utm.my/id/eprint/48793/ http://eprints.utm.my/id/eprint/48793/25/ChitraSelvaratnamMFS2014.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:83691 masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Selvaratnam, Chitra Identification and characterization of bacteria from the skin of jackfruit
description	Fresh-cut fruits industry is developing extensively in this global era due to busy lifestyle. However, the fresh-cut processing could be contaminated by microorganisms; resulting in food poisoning outbreaks. This study presented about the characterization and inhibition of bacteria isolated from the fresh skin of jackfruit. The isolation of single colonies was done by spread plate and streak plate method. The isolates were identified by 16S rRNA analysis. Gram staining, growth profiling and 15 biochemical tests were carried out to characterize each isolates.Lastly, the isolates were treated with various antimicrobial agents by disc agar diffusion technique and the fresh skin of jackfruit was treated with antimicrobialagent, XY-12 with different conditions by spread plate technique to study the effects of antimicrobial agents on microbial growth. Experimental results demonstrated that four types of bacterial single colonies were isolated with different morphology and designated as CE1, CE2, CE3 and CE4. The 16S rRNA analysis showed that the isolates of CE1 and CE4 were Bacillus sp. strain CY-b33. Isolates of CE2 and CE3were identified as Bacillus pumilus strain SBTBP-008 and Bacillus thuringiensis strain EA26.1 respectively. All the isolates were Gram positive and in rod shaped.The growth kinetics of CE3 was highest (0.2280 h-1) compared to CE1 (0.1317 h-1)which was the lowest. Bacteria CE1 and CE2 have the highest sensitivity againstXY-12 (0.6 mL/L) and Kanamycin Sulfate respectively. Bacteria CE3 and CE4 have the highest sensitivity against Ampicillin Trihydrate and Tetracycline Hydrochloride respectively. The fresh skin of jackfruit treated with XY-12 (0.6 mL/L) and incubated at 4°C revealed high efficiency in microbial reduction at day 2 (100%) and 4 (99.72%).
format	Thesis
qualification_level	Master's degree
author	Selvaratnam, Chitra
author_facet	Selvaratnam, Chitra
author_sort	Selvaratnam, Chitra
title	Identification and characterization of bacteria from the skin of jackfruit
title_short	Identification and characterization of bacteria from the skin of jackfruit
title_full	Identification and characterization of bacteria from the skin of jackfruit
title_fullStr	Identification and characterization of bacteria from the skin of jackfruit
title_full_unstemmed	Identification and characterization of bacteria from the skin of jackfruit
title_sort	identification and characterization of bacteria from the skin of jackfruit
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2014
url	http://eprints.utm.my/id/eprint/48793/25/ChitraSelvaratnamMFS2014.pdf
_version_	1747817473281884160

Identification and characterization of bacteria from the skin of jackfruit

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