Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning

Entomotoxicology deals with the identification and quantitation of drugs and poisons in maggots for the purpose of diagnosing the possible cause of death, especially in highly decomposed bodies wherein the conventional toxicological specimens are not suitable or available for sampling. In this conte...

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Main Author:	Kamarudin, Rosaniza
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/53611/1/RosanizaKamarudinMFS2015.pdf
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spelling	my-utm-ep.536112020-07-22T03:55:52Z Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning 2015-04 Kamarudin, Rosaniza Q Science (General) Entomotoxicology deals with the identification and quantitation of drugs and poisons in maggots for the purpose of diagnosing the possible cause of death, especially in highly decomposed bodies wherein the conventional toxicological specimens are not suitable or available for sampling. In this context, poisons such as paraquat have been reported as one of the agents used for committing suicides as well as accidental deaths and hence the use of Calliphorid maggots for toxicological analysis has been suggested. However, specific analytical method for analyzing paraquat in maggots remains unreported and in view of this problem, this present study that aimed to develop such method acquires forensic significance. The third instar of Calliphorid larvae that fed on paraquat-treated beef substrates in lethal dose for human (sample P1; 40 mg/kg) and three times the lethal dose (sample P2; 120 mg/kg) were collected and extracted using protein precipitation technique. Samples (0.4 g) were mixed with formic acid and ultrapure water, centrifuged and filtered. Paraquat residue was analysed using capillary zone electrophoresis with diode array detector (CZE-DAD). CZE analysis was performed in fused silica capillary under potential +25 kV and detection at 195 nm. The electrolyte was a 50 mM phosphate buffer at pH 2.50. Results of this study indicated that the presence of paraquat was detected in Calliphorid maggot samples that were collected from the paraquat-treated beef substrates in less than 2.5 mins under the optimized conditions and proved the reliability of these samples for qualitative analyses; LOD and LOQ were 0.495 µg/mL and 1.501 µg/mL, respectively. Detection of paraquat in maggots may prove useful in diagnosing the cause of death, since paraquat is a poison compared to other drugs of abuse. 2015-04 Thesis http://eprints.utm.my/id/eprint/53611/ http://eprints.utm.my/id/eprint/53611/1/RosanizaKamarudinMFS2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:85293 masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Kamarudin, Rosaniza Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning
description	Entomotoxicology deals with the identification and quantitation of drugs and poisons in maggots for the purpose of diagnosing the possible cause of death, especially in highly decomposed bodies wherein the conventional toxicological specimens are not suitable or available for sampling. In this context, poisons such as paraquat have been reported as one of the agents used for committing suicides as well as accidental deaths and hence the use of Calliphorid maggots for toxicological analysis has been suggested. However, specific analytical method for analyzing paraquat in maggots remains unreported and in view of this problem, this present study that aimed to develop such method acquires forensic significance. The third instar of Calliphorid larvae that fed on paraquat-treated beef substrates in lethal dose for human (sample P1; 40 mg/kg) and three times the lethal dose (sample P2; 120 mg/kg) were collected and extracted using protein precipitation technique. Samples (0.4 g) were mixed with formic acid and ultrapure water, centrifuged and filtered. Paraquat residue was analysed using capillary zone electrophoresis with diode array detector (CZE-DAD). CZE analysis was performed in fused silica capillary under potential +25 kV and detection at 195 nm. The electrolyte was a 50 mM phosphate buffer at pH 2.50. Results of this study indicated that the presence of paraquat was detected in Calliphorid maggot samples that were collected from the paraquat-treated beef substrates in less than 2.5 mins under the optimized conditions and proved the reliability of these samples for qualitative analyses; LOD and LOQ were 0.495 µg/mL and 1.501 µg/mL, respectively. Detection of paraquat in maggots may prove useful in diagnosing the cause of death, since paraquat is a poison compared to other drugs of abuse.
format	Thesis
qualification_level	Master's degree
author	Kamarudin, Rosaniza
author_facet	Kamarudin, Rosaniza
author_sort	Kamarudin, Rosaniza
title	Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning
title_short	Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning
title_full	Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning
title_fullStr	Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning
title_full_unstemmed	Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning
title_sort	detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2015
url	http://eprints.utm.my/id/eprint/53611/1/RosanizaKamarudinMFS2015.pdf
_version_	1747817594027507712

Detection of paraquat in calliphorid maggots by capillary electrophoresis for diagnosing fatal poisoning

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