Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells

Cervical cancer has one of the highest death recorded percentages among women in the world especially in less developed countries. Moreover, the presence of cancer stem cells in cervical cancer tumours is believed to cause resistance to conventional therapies. Previous studies have shown that C. nut...

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Main Author:	Abd. Rashid, Nor Syafiqah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	QH301 Biology
Online Access:	http://eprints.utm.my/id/eprint/78923/1/NorSyafiqahAbdMFBME2017.pdf
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id	my-utm-ep.78923
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spelling	my-utm-ep.789232018-09-17T07:23:15Z Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells 2017-07 Abd. Rashid, Nor Syafiqah QH301 Biology Cervical cancer has one of the highest death recorded percentages among women in the world especially in less developed countries. Moreover, the presence of cancer stem cells in cervical cancer tumours is believed to cause resistance to conventional therapies. Previous studies have shown that C. nutans extracts possess antioxidant and anti-proliferative effects towards several cervical cancer cell lines including HeLa cells. This study is focused at examining the anticancer effects of ethanolic C. nutans leave extract on cervical cancer cell viability, proliferation and its ability to induce apoptosis in cervical cancer cells. In order to achieve these objectives, the MTS, CyQuant and active caspase 3/7 assays as well as immunofluorescence microscopy analysis were conducted respectively on Hela cells which represent an invasive form of cervical cancer. Treatment of C. nutans leave extract at concentrations of 0-50 μg/mL showed a significant reduction in cell viability and proliferation in a dose dependent manner on the Hela cells. An IC50 of 40 μg/mL and 20 μg/mL C. nutans were observed respectively in inducing reduction in cell viability and proliferation. Furthermore, Hela cells treated with C. nutans resulted in a reduced expression level of CD133 cervical cancer stem cell maker compared to the untreated cells. A further reduction in cell proliferation compared to cell viability in Hela cells and reduced CD133 expression may suggest that C. nutans may specifically target cervical cancer stem cells. Additionally, an increased caspase-3/7 activity in Hela cells treated with C. nutans indicated that the plant extract induced apoptosis. Apoptosis was also demonstrated through change in Hela cell morphology and formation of apoptotic bodies upon C. nutans. Findings of this study suggest that C. nutans is a potential anticancer agent which can be used in adjuvant chemotherapy treatment of cervical cancer. 2017-07 Thesis http://eprints.utm.my/id/eprint/78923/ http://eprints.utm.my/id/eprint/78923/1/NorSyafiqahAbdMFBME2017.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:108246 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QH301 Biology
spellingShingle	QH301 Biology Abd. Rashid, Nor Syafiqah Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells
description	Cervical cancer has one of the highest death recorded percentages among women in the world especially in less developed countries. Moreover, the presence of cancer stem cells in cervical cancer tumours is believed to cause resistance to conventional therapies. Previous studies have shown that C. nutans extracts possess antioxidant and anti-proliferative effects towards several cervical cancer cell lines including HeLa cells. This study is focused at examining the anticancer effects of ethanolic C. nutans leave extract on cervical cancer cell viability, proliferation and its ability to induce apoptosis in cervical cancer cells. In order to achieve these objectives, the MTS, CyQuant and active caspase 3/7 assays as well as immunofluorescence microscopy analysis were conducted respectively on Hela cells which represent an invasive form of cervical cancer. Treatment of C. nutans leave extract at concentrations of 0-50 μg/mL showed a significant reduction in cell viability and proliferation in a dose dependent manner on the Hela cells. An IC50 of 40 μg/mL and 20 μg/mL C. nutans were observed respectively in inducing reduction in cell viability and proliferation. Furthermore, Hela cells treated with C. nutans resulted in a reduced expression level of CD133 cervical cancer stem cell maker compared to the untreated cells. A further reduction in cell proliferation compared to cell viability in Hela cells and reduced CD133 expression may suggest that C. nutans may specifically target cervical cancer stem cells. Additionally, an increased caspase-3/7 activity in Hela cells treated with C. nutans indicated that the plant extract induced apoptosis. Apoptosis was also demonstrated through change in Hela cell morphology and formation of apoptotic bodies upon C. nutans. Findings of this study suggest that C. nutans is a potential anticancer agent which can be used in adjuvant chemotherapy treatment of cervical cancer.
format	Thesis
qualification_level	Master's degree
author	Abd. Rashid, Nor Syafiqah
author_facet	Abd. Rashid, Nor Syafiqah
author_sort	Abd. Rashid, Nor Syafiqah
title	Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells
title_short	Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells
title_full	Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells
title_fullStr	Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells
title_full_unstemmed	Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells
title_sort	anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2017
url	http://eprints.utm.my/id/eprint/78923/1/NorSyafiqahAbdMFBME2017.pdf
_version_	1747818104634736640

Anticancer effects of clinacanthus nutans crude extracts in cervical cancer cells

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