Modified acrylamide hydrogel nanocomposites for varginal drug delivery system

This study conducted on the structure of modified acrylamide-based hydrogel by synthesizing the nano composites. The hydrogels employed in this study were provided through a combination of acrylamide monomers, montmorillonite clay, Sodium carboxymethylcellulose (NaCMC) and magnesium oxide (MgO) nano...

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Main Author: Mojaveryazdi, Farzaneh Sabbagh
Format: Thesis
Language:English
Published: 2017
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Online Access:http://eprints.utm.my/id/eprint/79292/1/FarzanehSabbaghMojaveryazdiPFChE2017.pdf
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spelling my-utm-ep.792922018-10-14T08:42:04Z Modified acrylamide hydrogel nanocomposites for varginal drug delivery system 2017 Mojaveryazdi, Farzaneh Sabbagh TP Chemical technology This study conducted on the structure of modified acrylamide-based hydrogel by synthesizing the nano composites. The hydrogels employed in this study were provided through a combination of acrylamide monomers, montmorillonite clay, Sodium carboxymethylcellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization. N, N, N’, N’-tetramethyl ethylenediamine and ammonium persulfate as the initiator were applied in the structure of the polymer. In addition, the total of the polymerization and characterization were utilized based on three types of hydrogels which are acrylamide (Aam), Aam/NaCMC and Aam/NaCMC/MgO hydrogels. The properties of surface morphology of the hydrogels were characterized by swelling ratio, field emission scanning electron microscope (FESEM), texture analysis, x-ray diffraction and Fourier transform infrared spectroscopy (FTIR). Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio and gel strength compared to Aam and Aam/NaCMC hydrogels. Thus, MgO is an appropriate nanoparticle to be used in the nano composites. The role of NaCMC was also studied in the swelling and consequently in drug release. The systems were characterized regarding rheological behavior of hydrogel, FTIR, and FESEM. The dispersion of the nanoparticles MgO and drug (acyclovir) inside the hydrogel was shown by transmission electron microscopy. Acyclovir, one of the famous drugs to treat the vaginal infections, was used as the drug for delivery and release in the vagina conditions. It was loaded into the polymer through the soaking method in an aqueous solution contained acyclovir. The drug release was studied in two different mediums, phosphate-buffer saline (PBS) (pH 7.4) and vaginal fluid simulant (SVF) (pH 4.5) aqueous solutions were utilized. The amount of released drug from the hydrogels was determined using high performance liquid chromatography. The best amount of NaCMC and MgO used in this study was 0.2 g and 0.01 g at pH 6, respectively. The aggregate percentage of released acyclovir diversed between 89.7% and 35.1% in SVF and 76.41% and 22.24% in PBS. In this study, the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay test showed that the acrylamide-based hydrogel demonstrated a cytotoxicity effect at 12.5, 25,50, 100 and 200 mg/ml concentrations. 2017 Thesis http://eprints.utm.my/id/eprint/79292/ http://eprints.utm.my/id/eprint/79292/1/FarzanehSabbaghMojaveryazdiPFChE2017.pdf application/pdf en public phd doctoral Universiti Teknologi Malaysia, Faculty of Chemical & Energy Engineering Faculty of Chemical & Energy Engineering
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mojaveryazdi, Farzaneh Sabbagh
Modified acrylamide hydrogel nanocomposites for varginal drug delivery system
description This study conducted on the structure of modified acrylamide-based hydrogel by synthesizing the nano composites. The hydrogels employed in this study were provided through a combination of acrylamide monomers, montmorillonite clay, Sodium carboxymethylcellulose (NaCMC) and magnesium oxide (MgO) nanoparticles by crosslinking polymerization. N, N, N’, N’-tetramethyl ethylenediamine and ammonium persulfate as the initiator were applied in the structure of the polymer. In addition, the total of the polymerization and characterization were utilized based on three types of hydrogels which are acrylamide (Aam), Aam/NaCMC and Aam/NaCMC/MgO hydrogels. The properties of surface morphology of the hydrogels were characterized by swelling ratio, field emission scanning electron microscope (FESEM), texture analysis, x-ray diffraction and Fourier transform infrared spectroscopy (FTIR). Findings of the study considered the nano composites consisting of MgO have the highest swelling ratio and gel strength compared to Aam and Aam/NaCMC hydrogels. Thus, MgO is an appropriate nanoparticle to be used in the nano composites. The role of NaCMC was also studied in the swelling and consequently in drug release. The systems were characterized regarding rheological behavior of hydrogel, FTIR, and FESEM. The dispersion of the nanoparticles MgO and drug (acyclovir) inside the hydrogel was shown by transmission electron microscopy. Acyclovir, one of the famous drugs to treat the vaginal infections, was used as the drug for delivery and release in the vagina conditions. It was loaded into the polymer through the soaking method in an aqueous solution contained acyclovir. The drug release was studied in two different mediums, phosphate-buffer saline (PBS) (pH 7.4) and vaginal fluid simulant (SVF) (pH 4.5) aqueous solutions were utilized. The amount of released drug from the hydrogels was determined using high performance liquid chromatography. The best amount of NaCMC and MgO used in this study was 0.2 g and 0.01 g at pH 6, respectively. The aggregate percentage of released acyclovir diversed between 89.7% and 35.1% in SVF and 76.41% and 22.24% in PBS. In this study, the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay test showed that the acrylamide-based hydrogel demonstrated a cytotoxicity effect at 12.5, 25,50, 100 and 200 mg/ml concentrations.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Mojaveryazdi, Farzaneh Sabbagh
author_facet Mojaveryazdi, Farzaneh Sabbagh
author_sort Mojaveryazdi, Farzaneh Sabbagh
title Modified acrylamide hydrogel nanocomposites for varginal drug delivery system
title_short Modified acrylamide hydrogel nanocomposites for varginal drug delivery system
title_full Modified acrylamide hydrogel nanocomposites for varginal drug delivery system
title_fullStr Modified acrylamide hydrogel nanocomposites for varginal drug delivery system
title_full_unstemmed Modified acrylamide hydrogel nanocomposites for varginal drug delivery system
title_sort modified acrylamide hydrogel nanocomposites for varginal drug delivery system
granting_institution Universiti Teknologi Malaysia, Faculty of Chemical & Energy Engineering
granting_department Faculty of Chemical & Energy Engineering
publishDate 2017
url http://eprints.utm.my/id/eprint/79292/1/FarzanehSabbaghMojaveryazdiPFChE2017.pdf
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