Identification of dehalogenase producing phychrotophic bacteria

2, 2 dichloropropionic acids (Dalapon) like most halogenated compounds are commonly used as herbicides and employed in agricultural areas and industries. Toxicity of these xenobiotic compounds causes serious environmental problems. Bacillus sp ih1 was isolated from top cliff soil collected from Anta...

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Main Author:	Haruna, Ismail
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH Natural history
Online Access:	http://eprints.utm.my/id/eprint/79326/1/IsmailHarunaMFBME2016.pdf
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id	my-utm-ep.79326
record_format	uketd_dc
spelling	my-utm-ep.793262018-10-14T08:44:25Z Identification of dehalogenase producing phychrotophic bacteria 2016 Haruna, Ismail QH Natural history 2, 2 dichloropropionic acids (Dalapon) like most halogenated compounds are commonly used as herbicides and employed in agricultural areas and industries. Toxicity of these xenobiotic compounds causes serious environmental problems. Bacillus sp ih1 was isolated from top cliff soil collected from Antarctica. The bacteria was first grown on Antarctic bacterial medium and later transferred to a minimal medium containing 2, 2, dichloropropionic acid as carbon source. It grew slowly in the minimal media in different concentrations of 10mM, 20mM, 30mM and 40mM of 2, 2 DCP. The best growth was observed in 20mM of 2, 2-DCP with doubling time 32hours. To monitor the degradation activity of the bacteria, halide ion assay was carried out to check the release of chloride ion. The best release of chloride was 0.657 mmol/L in 20mM of 2, 2-DCP. The bacteria was identification using 16S rRNA, genomic DNA extraction method and PCR amplification of 16S rRNA was performed using universal primers 27F and 1492R. Nucleotide blast (BLASTn) showed 97% similarity with bacillus sp. Results from biochemical tests further confirm the bacteria as bacillus sp. Using phylogeny.fr, sequences from nucleotide blast result were used to build a phylogeny tree based on neighbour to neighbour joining. 2016 Thesis http://eprints.utm.my/id/eprint/79326/ http://eprints.utm.my/id/eprint/79326/1/IsmailHarunaMFBME2016.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QH Natural history
spellingShingle	QH Natural history Haruna, Ismail Identification of dehalogenase producing phychrotophic bacteria
description	2, 2 dichloropropionic acids (Dalapon) like most halogenated compounds are commonly used as herbicides and employed in agricultural areas and industries. Toxicity of these xenobiotic compounds causes serious environmental problems. Bacillus sp ih1 was isolated from top cliff soil collected from Antarctica. The bacteria was first grown on Antarctic bacterial medium and later transferred to a minimal medium containing 2, 2, dichloropropionic acid as carbon source. It grew slowly in the minimal media in different concentrations of 10mM, 20mM, 30mM and 40mM of 2, 2 DCP. The best growth was observed in 20mM of 2, 2-DCP with doubling time 32hours. To monitor the degradation activity of the bacteria, halide ion assay was carried out to check the release of chloride ion. The best release of chloride was 0.657 mmol/L in 20mM of 2, 2-DCP. The bacteria was identification using 16S rRNA, genomic DNA extraction method and PCR amplification of 16S rRNA was performed using universal primers 27F and 1492R. Nucleotide blast (BLASTn) showed 97% similarity with bacillus sp. Results from biochemical tests further confirm the bacteria as bacillus sp. Using phylogeny.fr, sequences from nucleotide blast result were used to build a phylogeny tree based on neighbour to neighbour joining.
format	Thesis
qualification_level	Master's degree
author	Haruna, Ismail
author_facet	Haruna, Ismail
author_sort	Haruna, Ismail
title	Identification of dehalogenase producing phychrotophic bacteria
title_short	Identification of dehalogenase producing phychrotophic bacteria
title_full	Identification of dehalogenase producing phychrotophic bacteria
title_fullStr	Identification of dehalogenase producing phychrotophic bacteria
title_full_unstemmed	Identification of dehalogenase producing phychrotophic bacteria
title_sort	identification of dehalogenase producing phychrotophic bacteria
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/79326/1/IsmailHarunaMFBME2016.pdf
_version_	1747818201145671680

Identification of dehalogenase producing phychrotophic bacteria

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