Elastase and inflammation inhibitory from Labisia pumila

Labisia pumila (L. pumila) is widely reported to exhibit antioxidant, antimicrobialand anti-cancer activities. The goal of this study was to identify andquantify triterpenoid compounds from L. pumila leaves which are responsible foranti-inflammatory and anti-elastase activities by using bioassay-gui...

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Main Author:	Abdullah, Norhanisah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/79437/1/NorhanisahAbdullahPFChE2017.pdf
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spelling	my-utm-ep.794372018-10-21T04:34:45Z Elastase and inflammation inhibitory from Labisia pumila 2017 Abdullah, Norhanisah TP Chemical technology Labisia pumila (L. pumila) is widely reported to exhibit antioxidant, antimicrobialand anti-cancer activities. The goal of this study was to identify andquantify triterpenoid compounds from L. pumila leaves which are responsible foranti-inflammatory and anti-elastase activities by using bioassay-guided fractionation.The leaves of L. pumila were sequentially extracted with n-hexane, ethyl acetate,methanol, and water using serial exhaustive extraction procedure. Qualitativephytochemical analysis revealed the presence of phenolics, flavonoids, terpenoids,cardiac glycosides and saponins in the extracts. Each extract was assessed for totaltriterpenoids and polyphenols content by using vanillin-perchloric acid assay andFolin-Ciocalteu’s reagent, respectively. The ethyl acetate extract has the highest totaltriterpenoids content (78.94 mg oleanolic acid equivalent (OAE)/g dried extract) andappreciable amount of total polyphenols (38.93 mg gallic acid equivalent/g driedextract). The extracts were then tested for their anti-inflammatory and anti-elastaseactivities. The ethyl acetate extract showed the highest anti-inflammatory activity viathe inhibition of cyclooxygenase-2 (half-maximal inhibitory concentration (IC50) of34.32 μg/ml) and elastase (IC50 of 17.14 μg/ml). The correlation analysis of antiinflammatoryand anti-elastase ability of L. pumila with its total triterpenoids andtotal polyphenols content showed positive relationship. Further fractionation of theethyl acetate extract by solid phase extraction afforded sample fraction EAF-B withhigh total triterpenoids content (91.50 mg OAE/g dried fraction) and anti-elastaseactivity (IC50 of 13.27 μg/ml). Rapid liquid chromatography-electrospray ionizationtandemmass spectrometry method was developed and validated for the identificationand quantification of pentacyclic triterpenoids with elastase inhibition property fromthe sample fraction EAF-B. Three pentacyclic triterpenoids, namely betulinic acid,oleanolic acid and lupeol were identified. The content of betulinic acid, oleanolicacid and lupeol in the fraction were 2.67, 5.04 and 1.47 mg/g dried fraction,respectively. The limits of detection and quantification ranged from 0.04 to 0.12μg/ml and 0.11 to 0.65 μg/ml, respectively. The response surface methodologyanalysis showed that solvent-solid ratio (p<0.01), extraction time (p<0.05) andtemperature (p<0.01) were statistically significant factors affecting the totaltriterpenoids extraction. The optimum extraction conditions obtained were: solventsolidratio of 37.76, extraction time of 4.24 hours and extraction temperature of 55.27°C. The results suggested that L. pumila has promising anti-inflammatory and antielastaseactivity that could serve as potential source of natural elastase inhibitor intreating inflammatory skin disorders and photoaging. 2017 Thesis http://eprints.utm.my/id/eprint/79437/ http://eprints.utm.my/id/eprint/79437/1/NorhanisahAbdullahPFChE2017.pdf application/pdf en public phd doctoral Universiti Teknologi Malaysia, Faculty of Chemical and Energy Engineering Faculty of Chemical and Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Abdullah, Norhanisah Elastase and inflammation inhibitory from Labisia pumila
description	Labisia pumila (L. pumila) is widely reported to exhibit antioxidant, antimicrobialand anti-cancer activities. The goal of this study was to identify andquantify triterpenoid compounds from L. pumila leaves which are responsible foranti-inflammatory and anti-elastase activities by using bioassay-guided fractionation.The leaves of L. pumila were sequentially extracted with n-hexane, ethyl acetate,methanol, and water using serial exhaustive extraction procedure. Qualitativephytochemical analysis revealed the presence of phenolics, flavonoids, terpenoids,cardiac glycosides and saponins in the extracts. Each extract was assessed for totaltriterpenoids and polyphenols content by using vanillin-perchloric acid assay andFolin-Ciocalteu’s reagent, respectively. The ethyl acetate extract has the highest totaltriterpenoids content (78.94 mg oleanolic acid equivalent (OAE)/g dried extract) andappreciable amount of total polyphenols (38.93 mg gallic acid equivalent/g driedextract). The extracts were then tested for their anti-inflammatory and anti-elastaseactivities. The ethyl acetate extract showed the highest anti-inflammatory activity viathe inhibition of cyclooxygenase-2 (half-maximal inhibitory concentration (IC50) of34.32 μg/ml) and elastase (IC50 of 17.14 μg/ml). The correlation analysis of antiinflammatoryand anti-elastase ability of L. pumila with its total triterpenoids andtotal polyphenols content showed positive relationship. Further fractionation of theethyl acetate extract by solid phase extraction afforded sample fraction EAF-B withhigh total triterpenoids content (91.50 mg OAE/g dried fraction) and anti-elastaseactivity (IC50 of 13.27 μg/ml). Rapid liquid chromatography-electrospray ionizationtandemmass spectrometry method was developed and validated for the identificationand quantification of pentacyclic triterpenoids with elastase inhibition property fromthe sample fraction EAF-B. Three pentacyclic triterpenoids, namely betulinic acid,oleanolic acid and lupeol were identified. The content of betulinic acid, oleanolicacid and lupeol in the fraction were 2.67, 5.04 and 1.47 mg/g dried fraction,respectively. The limits of detection and quantification ranged from 0.04 to 0.12μg/ml and 0.11 to 0.65 μg/ml, respectively. The response surface methodologyanalysis showed that solvent-solid ratio (p<0.01), extraction time (p<0.05) andtemperature (p<0.01) were statistically significant factors affecting the totaltriterpenoids extraction. The optimum extraction conditions obtained were: solventsolidratio of 37.76, extraction time of 4.24 hours and extraction temperature of 55.27°C. The results suggested that L. pumila has promising anti-inflammatory and antielastaseactivity that could serve as potential source of natural elastase inhibitor intreating inflammatory skin disorders and photoaging.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Abdullah, Norhanisah
author_facet	Abdullah, Norhanisah
author_sort	Abdullah, Norhanisah
title	Elastase and inflammation inhibitory from Labisia pumila
title_short	Elastase and inflammation inhibitory from Labisia pumila
title_full	Elastase and inflammation inhibitory from Labisia pumila
title_fullStr	Elastase and inflammation inhibitory from Labisia pumila
title_full_unstemmed	Elastase and inflammation inhibitory from Labisia pumila
title_sort	elastase and inflammation inhibitory from labisia pumila
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical and Energy Engineering
granting_department	Faculty of Chemical and Energy Engineering
publishDate	2017
url	http://eprints.utm.my/id/eprint/79437/1/NorhanisahAbdullahPFChE2017.pdf
_version_	1747818227231096832

Elastase and inflammation inhibitory from Labisia pumila

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