Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2

Nowadays, 80% of the world energy requirement depends on fossil fuels. This ultimately will lead to a reduction in global fossil fuel resources. Hydrogen is a sustainable, clean source of energy and can be produced through biological process by microorganisms. The biohydrogen production is important...

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Main Author:	Ramli, Amalina
Format:	Thesis
Language:	English
Published:	2018
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/80885/1/AmalinaRamliMFS2018.pdf
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spelling	my-utm-ep.808852019-07-24T00:10:15Z Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2 2018-08 Ramli, Amalina TP Chemical technology Nowadays, 80% of the world energy requirement depends on fossil fuels. This ultimately will lead to a reduction in global fossil fuel resources. Hydrogen is a sustainable, clean source of energy and can be produced through biological process by microorganisms. The biohydrogen production is important due to both environmental and economic factors, since waste substrates can be used to generate biogas rich in biohydrogen. The aim of this study was to isolate biohydrogen producing bacteria from agricultural wastewater and to optimise the biohydrogen production using enrichment medium. Out of 25 bacteria obtained, only 13 isolates had the ability to produce biohydrogen. One isolate that produced the highest biohydrogen was selected for 16S rDNA sequence identification. The bacterium was identified as Klebsiella sp. strain PR2. For the optimisation of biohydrogen production, fermentation was carried out in batch culture, where parameters such as carbon and nitrogen source concentration, initial pH of the medium, inoculum size, as well as incubation temperature were investigated. D-mannitol was found to be the best carbon source while peptone was the best nitrogen source for biohydrogen production with concentration of 0.2% (w/v) and 0.5% (w/v), respectively. The optimum condition for biohydrogen production was achieved at the initial pH 8 of the medium, incubation temperature of 30 °C and 10% (v/v) of inoculum. After 24 hours of incubation, the highest yield obtained (YP/X) was 8547.42 g/g while the highest biohydrogen productivity was 177.24 mol/L/h. Biohydrogen production using palm oil mill effluent (POME) as substrate was also conducted. Fermentation was carried out in sterile raw and final discharge POME by inoculating with 10% (v/v) bacteria inoculum and incubated at 30 oC for 5 days. The maximum biohydrogen productivity from raw and final discharge POME were 1.44 mol/L/h and 1.81 mol/L/h, respectively. The highest colour removal were 38% (initial: 18167 ADMI) for raw discharge POME and 31% (initial: 1873 ADMI) for final discharge POME were obtained after 5 days of treatment. Meanwhile, the highest COD removal were 34.8% (initial: 7320 mg/L) and 50.8% (initial: 1410 mg/L) for the raw and final discharge POME, respectively. From this study, it could be concluded that Klebsiella sp. strain PR2 has potential applications for biohydrogen production utilising POME as substrate besides treating the POME through biodegradation process. 2018-08 Thesis http://eprints.utm.my/id/eprint/80885/ http://eprints.utm.my/id/eprint/80885/1/AmalinaRamliMFS2018.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:124861 masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ramli, Amalina Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2
description	Nowadays, 80% of the world energy requirement depends on fossil fuels. This ultimately will lead to a reduction in global fossil fuel resources. Hydrogen is a sustainable, clean source of energy and can be produced through biological process by microorganisms. The biohydrogen production is important due to both environmental and economic factors, since waste substrates can be used to generate biogas rich in biohydrogen. The aim of this study was to isolate biohydrogen producing bacteria from agricultural wastewater and to optimise the biohydrogen production using enrichment medium. Out of 25 bacteria obtained, only 13 isolates had the ability to produce biohydrogen. One isolate that produced the highest biohydrogen was selected for 16S rDNA sequence identification. The bacterium was identified as Klebsiella sp. strain PR2. For the optimisation of biohydrogen production, fermentation was carried out in batch culture, where parameters such as carbon and nitrogen source concentration, initial pH of the medium, inoculum size, as well as incubation temperature were investigated. D-mannitol was found to be the best carbon source while peptone was the best nitrogen source for biohydrogen production with concentration of 0.2% (w/v) and 0.5% (w/v), respectively. The optimum condition for biohydrogen production was achieved at the initial pH 8 of the medium, incubation temperature of 30 °C and 10% (v/v) of inoculum. After 24 hours of incubation, the highest yield obtained (YP/X) was 8547.42 g/g while the highest biohydrogen productivity was 177.24 mol/L/h. Biohydrogen production using palm oil mill effluent (POME) as substrate was also conducted. Fermentation was carried out in sterile raw and final discharge POME by inoculating with 10% (v/v) bacteria inoculum and incubated at 30 oC for 5 days. The maximum biohydrogen productivity from raw and final discharge POME were 1.44 mol/L/h and 1.81 mol/L/h, respectively. The highest colour removal were 38% (initial: 18167 ADMI) for raw discharge POME and 31% (initial: 1873 ADMI) for final discharge POME were obtained after 5 days of treatment. Meanwhile, the highest COD removal were 34.8% (initial: 7320 mg/L) and 50.8% (initial: 1410 mg/L) for the raw and final discharge POME, respectively. From this study, it could be concluded that Klebsiella sp. strain PR2 has potential applications for biohydrogen production utilising POME as substrate besides treating the POME through biodegradation process.
format	Thesis
qualification_level	Master's degree
author	Ramli, Amalina
author_facet	Ramli, Amalina
author_sort	Ramli, Amalina
title	Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2
title_short	Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2
title_full	Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2
title_fullStr	Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2
title_full_unstemmed	Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2
title_sort	optimisation of biohydrogen production by locally isolated klebsiella sp. strain pr2
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2018
url	http://eprints.utm.my/id/eprint/80885/1/AmalinaRamliMFS2018.pdf
_version_	1747818274482028544

Optimisation of biohydrogen production by locally isolated Klebsiella sp. strain PR2

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