Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods

Over the past few years, there have been a rapid development in forensic drug testing in biological specimen. Urine and blood are the most commonly used biological matrices for detection of cocaine. However, there are some disadvantages such as invasive to the privacy and personal safety of the dono...

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Main Author:	Abdul Latheef, Mohamed Inas
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/81611/1/MohamedInasAbdulMFS2019.pdf
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spelling	my-utm-ep.816112019-09-10T01:49:59Z Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods 2019 Abdul Latheef, Mohamed Inas Q Science (General) Over the past few years, there have been a rapid development in forensic drug testing in biological specimen. Urine and blood are the most commonly used biological matrices for detection of cocaine. However, there are some disadvantages such as invasive to the privacy and personal safety of the donor as well as the instability of the cocaine in that biological matrix. Therefore, in this study, saliva has been proposed as an alternative matrix since the collection of saliva is non-invasive and rapid. In this study, the liquid-liquid extraction (LLE) method was utilized to extract cocaine from saliva before the analysis was done using optimized conditions of gas chromatography and mass selective detector (GC-MSD). Diethylether: isopropanol with volume to solvent ratio 3:5 was used in this study. The optimized method was validated by evaluating calibration curve, linearity, limit of detection (LOD), limit of quantification (LOQ), percentage recovery and intra-assay and inter-assay precision accuracy. From the calibration curve (0.5-10 ppm), good coefficient of determination was obtained at r² = 0.9994. LOD and LOQ were obtained at 0.29 and 0.97 ppm respectively. From the precision and accuracy analysis of intra and inter-day, lower coefficient variance (intra: 1.96%; inter: 0.79%) and high accuracy were attained (intra: 102.33%; inter: 101.20%). A mean recovery of 85.20% with acceptable coefficient of variance (CV < 6%) were also acquired. Finally, the stability of cocaine in the saliva were studied for 13 days. The analysis found that cocaine can be recovered with a mean recovery > 70 % without addition of any preservatives or buffers. The study showed that the analysis of cocaine in saliva is robust, hence it can be used as an alternative matrix for forensic drug testing. 2019 Thesis http://eprints.utm.my/id/eprint/81611/ http://eprints.utm.my/id/eprint/81611/1/MohamedInasAbdulMFS2019.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:124998 masters Universiti Teknologi Malaysia Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Abdul Latheef, Mohamed Inas Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods
description	Over the past few years, there have been a rapid development in forensic drug testing in biological specimen. Urine and blood are the most commonly used biological matrices for detection of cocaine. However, there are some disadvantages such as invasive to the privacy and personal safety of the donor as well as the instability of the cocaine in that biological matrix. Therefore, in this study, saliva has been proposed as an alternative matrix since the collection of saliva is non-invasive and rapid. In this study, the liquid-liquid extraction (LLE) method was utilized to extract cocaine from saliva before the analysis was done using optimized conditions of gas chromatography and mass selective detector (GC-MSD). Diethylether: isopropanol with volume to solvent ratio 3:5 was used in this study. The optimized method was validated by evaluating calibration curve, linearity, limit of detection (LOD), limit of quantification (LOQ), percentage recovery and intra-assay and inter-assay precision accuracy. From the calibration curve (0.5-10 ppm), good coefficient of determination was obtained at r² = 0.9994. LOD and LOQ were obtained at 0.29 and 0.97 ppm respectively. From the precision and accuracy analysis of intra and inter-day, lower coefficient variance (intra: 1.96%; inter: 0.79%) and high accuracy were attained (intra: 102.33%; inter: 101.20%). A mean recovery of 85.20% with acceptable coefficient of variance (CV < 6%) were also acquired. Finally, the stability of cocaine in the saliva were studied for 13 days. The analysis found that cocaine can be recovered with a mean recovery > 70 % without addition of any preservatives or buffers. The study showed that the analysis of cocaine in saliva is robust, hence it can be used as an alternative matrix for forensic drug testing.
format	Thesis
qualification_level	Master's degree
author	Abdul Latheef, Mohamed Inas
author_facet	Abdul Latheef, Mohamed Inas
author_sort	Abdul Latheef, Mohamed Inas
title	Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods
title_short	Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods
title_full	Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods
title_fullStr	Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods
title_full_unstemmed	Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods
title_sort	determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods
granting_institution	Universiti Teknologi Malaysia
granting_department	Biosciences and Medical Engineering
publishDate	2019
url	http://eprints.utm.my/id/eprint/81611/1/MohamedInasAbdulMFS2019.pdf
_version_	1747818370802122752

Determination of cocaine in spiked saliva using liquid-liquid extraction and gas chromatography- mass selective detector methods

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