Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer

Body weight loss strategies include combination of nutrition consultation, regular exercise, drug prescription, invasive intervention and/or non-invasive intervention. A non-invasive intervention such as transdermal administration of active ingredients helps in reducing localized subcutaneous adipos...

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Main Author:	Rosli, Nur Ayshah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/86204/1/NurAyshahRosliPSChE2019.pdf
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spelling	my-utm-ep.862042020-08-30T09:05:18Z Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer 2019 Rosli, Nur Ayshah TP Chemical technology Body weight loss strategies include combination of nutrition consultation, regular exercise, drug prescription, invasive intervention and/or non-invasive intervention. A non-invasive intervention such as transdermal administration of active ingredients helps in reducing localized subcutaneous adipose tissue through lipolysis. Zingiber officinale (ZO) and Zingiber zerumbet (ZZ) belong to the Zingiberaceae family and both have been discovered to possess lipolysis activity. An efficient drug delivery system is required to encapsulate ZO and ZZ, and deliver these substances up to the subcutaneous skin layer. In this study, ZO and ZZ oil were successfully encapsulated in nanostructured lipid carrier (NLC) using hot homogenization technique followed by ultrasonication. D-optimal mixture design was used to optimize the NLC in which the composition of ZO and ZZ oil, solid lipid, and liquid lipid were the independent variables, while particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency were the dependent variables. From the study, the optimum formulation for NLC-ZO was 3.6% ZO oil, 1.4% glyceryl monostearate, and 5.0% virgin coconut oil, and the NLC-ZO obtained had the following properties: 90.7 nm particle size, 0.15 PDI, -45.7 mV zeta potential and 88.8% encapsulation efficiency. The optimum NLC-ZZ formulation was 3.7% ZZ oil, 1.3% glyceryl monostearate, and 5.0% virgin coconut oil, and the NLC-ZZ obtained had the following properties: 91.0 nm particle size, 0.17 PDI, -40.9 mV zeta potential and 94.4% encapsulation efficiency. Morphology study revealed the spherical shape of NLC-ZO and NLC-ZZ, and the particle size obtained through transmission electron microscope conformed with the size measured through dynamic light scattering technique. Thermogram profile of NLC showed incorporation of ZO and ZZ into NLC lowered the melting temperature of lipid mixture, thus producing a less ordered crystalline structure of NLC to accommodate the active ingredients. Fourier transform infrared spectroscopy analysis demonstrated an interaction existed between ZO/ZZ and the NLC system. In vitro penetration study using Strat-M® membrane and freshly excised rat skin showed NLC-ZO and NLC-ZZ had higher penetration flux compared to free ZO and ZZ. The release of ZO from NLC-ZO followed Korsmeyer-Peppas model, and the release of ZZ from NLC-ZZ followed zero order kinetic model. 90-day storage stability study showed no significant changes for both NLC-ZO and NLC-ZZ in terms of particle size, PDI and zeta potential. In vitro lipolysis study revealed the potential of NLC-ZO and NLC-ZZ as anti-obesity agents as they stimulated the release of glycerol in 3T3-L1 adipocytes cells. In vivo NLC distribution in female Sprague- Dawley rats showed penetration deep into the dermis layer after 12 h of application. Free fatty acid release was detected when the rats were treated with NLC-ZO and NLC-ZZ for 3 h. This study demonstrated that ZO and ZZ were successfully encapsulated in NLC and the nanoparticles were effectively utilized to enhance dermis penetration and induce lipolysis activity via in vitro and in vivo. 2019 Thesis http://eprints.utm.my/id/eprint/86204/ http://eprints.utm.my/id/eprint/86204/1/NurAyshahRosliPSChE2019.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:131566 phd doctoral Universiti Teknologi Malaysia, Faculty of Engineering - School of Chemical & Energy Engineering Faculty of Engineering - School of Chemical & Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rosli, Nur Ayshah Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer
description	Body weight loss strategies include combination of nutrition consultation, regular exercise, drug prescription, invasive intervention and/or non-invasive intervention. A non-invasive intervention such as transdermal administration of active ingredients helps in reducing localized subcutaneous adipose tissue through lipolysis. Zingiber officinale (ZO) and Zingiber zerumbet (ZZ) belong to the Zingiberaceae family and both have been discovered to possess lipolysis activity. An efficient drug delivery system is required to encapsulate ZO and ZZ, and deliver these substances up to the subcutaneous skin layer. In this study, ZO and ZZ oil were successfully encapsulated in nanostructured lipid carrier (NLC) using hot homogenization technique followed by ultrasonication. D-optimal mixture design was used to optimize the NLC in which the composition of ZO and ZZ oil, solid lipid, and liquid lipid were the independent variables, while particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency were the dependent variables. From the study, the optimum formulation for NLC-ZO was 3.6% ZO oil, 1.4% glyceryl monostearate, and 5.0% virgin coconut oil, and the NLC-ZO obtained had the following properties: 90.7 nm particle size, 0.15 PDI, -45.7 mV zeta potential and 88.8% encapsulation efficiency. The optimum NLC-ZZ formulation was 3.7% ZZ oil, 1.3% glyceryl monostearate, and 5.0% virgin coconut oil, and the NLC-ZZ obtained had the following properties: 91.0 nm particle size, 0.17 PDI, -40.9 mV zeta potential and 94.4% encapsulation efficiency. Morphology study revealed the spherical shape of NLC-ZO and NLC-ZZ, and the particle size obtained through transmission electron microscope conformed with the size measured through dynamic light scattering technique. Thermogram profile of NLC showed incorporation of ZO and ZZ into NLC lowered the melting temperature of lipid mixture, thus producing a less ordered crystalline structure of NLC to accommodate the active ingredients. Fourier transform infrared spectroscopy analysis demonstrated an interaction existed between ZO/ZZ and the NLC system. In vitro penetration study using Strat-M® membrane and freshly excised rat skin showed NLC-ZO and NLC-ZZ had higher penetration flux compared to free ZO and ZZ. The release of ZO from NLC-ZO followed Korsmeyer-Peppas model, and the release of ZZ from NLC-ZZ followed zero order kinetic model. 90-day storage stability study showed no significant changes for both NLC-ZO and NLC-ZZ in terms of particle size, PDI and zeta potential. In vitro lipolysis study revealed the potential of NLC-ZO and NLC-ZZ as anti-obesity agents as they stimulated the release of glycerol in 3T3-L1 adipocytes cells. In vivo NLC distribution in female Sprague- Dawley rats showed penetration deep into the dermis layer after 12 h of application. Free fatty acid release was detected when the rats were treated with NLC-ZO and NLC-ZZ for 3 h. This study demonstrated that ZO and ZZ were successfully encapsulated in NLC and the nanoparticles were effectively utilized to enhance dermis penetration and induce lipolysis activity via in vitro and in vivo.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Rosli, Nur Ayshah
author_facet	Rosli, Nur Ayshah
author_sort	Rosli, Nur Ayshah
title	Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer
title_short	Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer
title_full	Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer
title_fullStr	Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer
title_full_unstemmed	Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer
title_sort	nanostructured lipid carrier loaded with zingiber officinale and zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer
granting_institution	Universiti Teknologi Malaysia, Faculty of Engineering - School of Chemical & Energy Engineering
granting_department	Faculty of Engineering - School of Chemical & Energy Engineering
publishDate	2019
url	http://eprints.utm.my/id/eprint/86204/1/NurAyshahRosliPSChE2019.pdf
_version_	1747818504112832512

Nanostructured lipid carrier loaded with zingiber officinale and Zingiber zerumbet oil for induction of lipolysis in subcutaneous skin layer

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