Marine microbial diversity of off-Terengganu coastal sediment in South China Sea

Marine bacteria play a vital role in regulating global biochemical cycle for billions of years, and their function has been widely explored for the past fifty years. Marine bacteria exploration is considered as difficult and precarious, but every finding is fruitful in providing information to gener...

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Main Author:	Zahar, Marziah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	TC Hydraulic engineering Ocean engineering
Online Access:	http://eprints.utm.my/id/eprint/92564/1/MarziahZaharPMJIIT2017.pdf
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id	my-utm-ep.92564
record_format	uketd_dc
spelling	my-utm-ep.925642021-09-30T15:11:40Z Marine microbial diversity of off-Terengganu coastal sediment in South China Sea 2017 Zahar, Marziah TC Hydraulic engineering. Ocean engineering Marine bacteria play a vital role in regulating global biochemical cycle for billions of years, and their function has been widely explored for the past fifty years. Marine bacteria exploration is considered as difficult and precarious, but every finding is fruitful in providing information to generate a better understanding of its purpose in the seawater. Marine bacteria exploration in Malaysia coastline is considered as new with no impactful data to represent the bacteria distribution in Malaysia’s coastline, specifically heading towards the South China Sea. The purpose of this study is to assess bacteria diversity off-Terengganu coast as the foremost marine bacteria abundance screening in these areas. In this study, surface sea sediment that contains a variety of bacteria cells is collected in three random locations with three different depths. The DNA obtained from the cell extraction was identified with Next Generation Sequence method, which specifically targeted 16SrDNA V3-V4 properties to obtain the overall bacterial metagenomic profile. Results showed that off-Terengganu coast, bacteria diversity consisted of 25518 amplicons of 3301 unique OTUs, which signify 27 phyla. The OTU abundance decreased gradually with depth of sediment in the sea. The metagenomic profile revealed two sulphur-degrading bacteria were dominant in the surveyed area. Sulfurovum genus dominate overall bacteria community in two locations situated in the northeast area of sampling stations. Conversely, Pseudoalteromonas dominated the overall bacterial community in the southeast coastline. The Physical-geochemical analysis revealed that all surveyed areas contained sulphur, oil, grease, gasoline, diesel, and mineral oil, which perhaps are influencing sulphur-degraded bacteria community growth in the surveyed area. There is no concrete evidence to link Sulfurovum and Pseudoalteromonas as pathogenic bacteria that causes illness to the human. However, there are possibility that the surveyed areas are anthropogenically polluted and further physical-geochemical analysis is required. In conclusion, the research findings suggested the necessity to conduct a broader bacteria diversity research, such as bacterial dispersion scale, and community variation in order to measure an inordinate extent of environmental pollution in the surveyed areas. 2017 Thesis http://eprints.utm.my/id/eprint/92564/ http://eprints.utm.my/id/eprint/92564/1/MarziahZaharPMJIIT2017.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:132795 phd doctoral Universiti Teknologi Malaysia Malaysia-Japan International Institute of Technology (MJIIT)
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TC Hydraulic engineering Ocean engineering
spellingShingle	TC Hydraulic engineering Ocean engineering Zahar, Marziah Marine microbial diversity of off-Terengganu coastal sediment in South China Sea
description	Marine bacteria play a vital role in regulating global biochemical cycle for billions of years, and their function has been widely explored for the past fifty years. Marine bacteria exploration is considered as difficult and precarious, but every finding is fruitful in providing information to generate a better understanding of its purpose in the seawater. Marine bacteria exploration in Malaysia coastline is considered as new with no impactful data to represent the bacteria distribution in Malaysia’s coastline, specifically heading towards the South China Sea. The purpose of this study is to assess bacteria diversity off-Terengganu coast as the foremost marine bacteria abundance screening in these areas. In this study, surface sea sediment that contains a variety of bacteria cells is collected in three random locations with three different depths. The DNA obtained from the cell extraction was identified with Next Generation Sequence method, which specifically targeted 16SrDNA V3-V4 properties to obtain the overall bacterial metagenomic profile. Results showed that off-Terengganu coast, bacteria diversity consisted of 25518 amplicons of 3301 unique OTUs, which signify 27 phyla. The OTU abundance decreased gradually with depth of sediment in the sea. The metagenomic profile revealed two sulphur-degrading bacteria were dominant in the surveyed area. Sulfurovum genus dominate overall bacteria community in two locations situated in the northeast area of sampling stations. Conversely, Pseudoalteromonas dominated the overall bacterial community in the southeast coastline. The Physical-geochemical analysis revealed that all surveyed areas contained sulphur, oil, grease, gasoline, diesel, and mineral oil, which perhaps are influencing sulphur-degraded bacteria community growth in the surveyed area. There is no concrete evidence to link Sulfurovum and Pseudoalteromonas as pathogenic bacteria that causes illness to the human. However, there are possibility that the surveyed areas are anthropogenically polluted and further physical-geochemical analysis is required. In conclusion, the research findings suggested the necessity to conduct a broader bacteria diversity research, such as bacterial dispersion scale, and community variation in order to measure an inordinate extent of environmental pollution in the surveyed areas.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Zahar, Marziah
author_facet	Zahar, Marziah
author_sort	Zahar, Marziah
title	Marine microbial diversity of off-Terengganu coastal sediment in South China Sea
title_short	Marine microbial diversity of off-Terengganu coastal sediment in South China Sea
title_full	Marine microbial diversity of off-Terengganu coastal sediment in South China Sea
title_fullStr	Marine microbial diversity of off-Terengganu coastal sediment in South China Sea
title_full_unstemmed	Marine microbial diversity of off-Terengganu coastal sediment in South China Sea
title_sort	marine microbial diversity of off-terengganu coastal sediment in south china sea
granting_institution	Universiti Teknologi Malaysia
granting_department	Malaysia-Japan International Institute of Technology (MJIIT)
publishDate	2017
url	http://eprints.utm.my/id/eprint/92564/1/MarziahZaharPMJIIT2017.pdf
_version_	1747818604509790208

Marine microbial diversity of off-Terengganu coastal sediment in South China Sea

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