Fish scale hydroxyapatite/collagen composites incorporated with silver nanoparticles for bone filler applications
<p>The aim of this study was to improve antimicrobial properties of fish scales</p><p>hydroxyapatite/fish collagen (FsHA/FsCol) composites through infiltration of silver nanoparticles</p><p>into the matrix for antimicrobial bone f...
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| Format: | thesis |
| Language: | eng |
| Published: |
2021
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=11638 |
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| Summary: | <p>The aim of this study was to improve antimicrobial properties of fish scales</p><p>hydroxyapatite/fish collagen (FsHA/FsCol) composites through infiltration of silver nanoparticles</p><p>into the matrix for antimicrobial bone filller substitute. In this sudy both hydroxyapatite and</p><p>collagen were extracted from fish scales using simple hydrothermal extraction method. Silver</p><p>nanoparticles (AgNPs) were synthesized from silver nitrate using microwave-assisted method in which</p><p>neems leaves extract was used as reducing agent while fish collagen as stabilizer. FsHA/FsCol/AgNPs</p><p>composites were prepared in a form of powders and beads using two different methods. Chemical and</p><p>physical properties of AgNPs and FsHA/FsCol/AgNPs composites were characterized using field</p><p>emmision scanning electron microscope with energy dispersive x-ray (FESEM-EDX), transmission</p><p>electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and x-ray</p><p>diffraction technique (XRD). Biological properties of prepared composites were investigated</p><p>through antibacterial, cytotoxicity and cell attachment tests. The results shown that four</p><p>different sizes for AgNPs ranging from 28 nm to I 00 nm were obtained by using different</p><p>concentration of collagen. Their antimicrobial activities against Escherichia coli and</p><p>Staphylococcus aureus showed that smaller particles sizes of AgNPs have shown better inhibition</p><p>ofbacteria growth. Analysis using XRD and EDX proved that the pattern of spectra corresponded to</p><p>silver nanoparticles present in the composites. FESEM-EDX analysis indicated that the silver</p><p>nanoparticles distributed well in the HA composite matrix. Composites with 80 wt.% of HA and 20</p><p>wt.% of collagen demonstrated high stability during degradation test. The addition of 20 wt.%</p><p>starch in the composite during preparation improved the porosity of composites after sintering,</p><p>thus improved the infiltration of AgNPs-collagen solution and antibacterial properties. In</p><p>conclusion, composites ofFsHA/FsCol/AgNPs powder and beads have been proved to be biocompatible and</p><p>good cell attachment. The implication ofthis study is both powder and beads of FsHA/FsCol/AgNPs</p><p>composties can be used as antimicrobial bone filler substitute.</p><p></p> |
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