Biomass production of local microalgae isolates in nitrified landfill leachate (IR)

Biomass production of local microalgae isolates in nitrified landfill leachate (IR)

This study aims to examine phycoremediation and biomass production of local microalgae isolates cultivated in high nitrate concentration of nitrified landfill leachate (NLL). The microalgae samples were collected from landfill leachate treatment plant and were isolated as potential species that are...

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Main Author: Norazela Nordin
Format: thesis
Language:eng
Published: 2016
Subjects:
Q Science (General)
Online Access:https://ir.upsi.edu.my/detailsg.php?det=2833
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spelling oai:ir.upsi.edu.my:28332020-02-27 Biomass production of local microalgae isolates in nitrified landfill leachate (IR) 2016 Norazela Nordin Q Science (General) This study aims to examine phycoremediation and biomass production of local microalgae isolates cultivated in high nitrate concentration of nitrified landfill leachate (NLL). The microalgae samples were collected from landfill leachate treatment plant and were isolated as potential species that are able to grow in high nitrate concentration. The isolates were identified using APHA's Key for Identification of Freshwater Algae and 16S rRNAl18S rDNA molecular techniques. Maximum microalgae growth, nitrate removal performance, and biomass productivity were determined at NLL concentration of 10 30% v/v. Biomass produced was further characterized for carbohydrate, lipid, and protein composition. The findings indicated that three species of local microalgae were successfully isolated namely; Chlorella sp., Scenedesmus sp. and Oscillatoria sp. with highest specific growth rate (11) of 0.62 day" (15% NLL), 0.71 day" (10% NLL), and 0.94 day" (10% NLL), respectively. Nitrate was removed by all microalgae isolates with nitrate removal rate of 26.5-27.5 mgl.i'day" and maximum nitrate removal percentage of84% (20% NLL) by Oscillatoria sp. Highest biomass productivity (106.87 mgl.i'day"), carbohydrate productivity (2921.83 mgl.t'day"), lipid productivity (1414.96 mgl.i'day") were observed for Oscillatoria sp. cultured in 10% NLL. Highest protein productivity (5498.37 mgl.i'day") was observed for Oscillatoria sp. cultured in 20% NLL. Highest carbohydrate, lipid, and protein content were observed for Chlorella sp. cultured in 30% NLL (37.50%), Chlorella sp. cultured in 10% NLL (18.23%), and Oscillatoria sp. cultured in 20% NLL (61.4%), respectively. The highest carbohydrate, lipid, and protein yield were achieved by Oscillatoria sp. cultured in 10% NLL (0.95 mg carbohydrate mg" NOJ-), Chlorella sp. cultured in 10% NLL (0.5 mg lipid mg' N03"), and Oscillatoria sp. cultured in 10% NLL (1.58 mg protein rng' N031 respectively. In conclusion, application of local microalgae isolates in phycoremediation of NLL and biomass production was feasible. As implication, local microalgae isolates performance in this research could serves as a basis for scaling up of microalgae biomass production. 2016 thesis https://ir.upsi.edu.my/detailsg.php?det=2833 https://ir.upsi.edu.my/detailsg.php?det=2833 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik N/A
institution Universiti Pendidikan Sultan Idris
collection UPSI Digital Repository
language eng
topic Q Science (General)
spellingShingle Q Science (General)
Norazela Nordin
Biomass production of local microalgae isolates in nitrified landfill leachate (IR)
description This study aims to examine phycoremediation and biomass production of local microalgae isolates cultivated in high nitrate concentration of nitrified landfill leachate (NLL). The microalgae samples were collected from landfill leachate treatment plant and were isolated as potential species that are able to grow in high nitrate concentration. The isolates were identified using APHA's Key for Identification of Freshwater Algae and 16S rRNAl18S rDNA molecular techniques. Maximum microalgae growth, nitrate removal performance, and biomass productivity were determined at NLL concentration of 10 30% v/v. Biomass produced was further characterized for carbohydrate, lipid, and protein composition. The findings indicated that three species of local microalgae were successfully isolated namely; Chlorella sp., Scenedesmus sp. and Oscillatoria sp. with highest specific growth rate (11) of 0.62 day" (15% NLL), 0.71 day" (10% NLL), and 0.94 day" (10% NLL), respectively. Nitrate was removed by all microalgae isolates with nitrate removal rate of 26.5-27.5 mgl.i'day" and maximum nitrate removal percentage of84% (20% NLL) by Oscillatoria sp. Highest biomass productivity (106.87 mgl.i'day"), carbohydrate productivity (2921.83 mgl.t'day"), lipid productivity (1414.96 mgl.i'day") were observed for Oscillatoria sp. cultured in 10% NLL. Highest protein productivity (5498.37 mgl.i'day") was observed for Oscillatoria sp. cultured in 20% NLL. Highest carbohydrate, lipid, and protein content were observed for Chlorella sp. cultured in 30% NLL (37.50%), Chlorella sp. cultured in 10% NLL (18.23%), and Oscillatoria sp. cultured in 20% NLL (61.4%), respectively. The highest carbohydrate, lipid, and protein yield were achieved by Oscillatoria sp. cultured in 10% NLL (0.95 mg carbohydrate mg" NOJ-), Chlorella sp. cultured in 10% NLL (0.5 mg lipid mg' N03"), and Oscillatoria sp. cultured in 10% NLL (1.58 mg protein rng' N031 respectively. In conclusion, application of local microalgae isolates in phycoremediation of NLL and biomass production was feasible. As implication, local microalgae isolates performance in this research could serves as a basis for scaling up of microalgae biomass production.
format thesis
qualification_name
qualification_level Master's degree
author Norazela Nordin
author_facet Norazela Nordin
author_sort Norazela Nordin
title Biomass production of local microalgae isolates in nitrified landfill leachate (IR)
title_short Biomass production of local microalgae isolates in nitrified landfill leachate (IR)
title_full Biomass production of local microalgae isolates in nitrified landfill leachate (IR)
title_fullStr Biomass production of local microalgae isolates in nitrified landfill leachate (IR)
title_full_unstemmed Biomass production of local microalgae isolates in nitrified landfill leachate (IR)
title_sort biomass production of local microalgae isolates in nitrified landfill leachate (ir)
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Sains dan Matematik
publishDate 2016
url https://ir.upsi.edu.my/detailsg.php?det=2833
_version_ 1747833037642530816

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