In vitro regeneration and acclimatization of Carica papaya L. (IR)
The purpose of this reseach is to study in vitro organogenesis of Carica papaya L. through tissue culture system. In vitro plant regeneration, production of synthetic seeds and acclimatization of plantlets were investigated. The experimental design that was used in this research is Completely Random...
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| Format: | thesis |
| Language: | eng |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=3811 |
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| Summary: | The purpose of this reseach is to study in vitro organogenesis of Carica papaya L. through tissue culture system. In vitro plant regeneration, production of synthetic seeds and acclimatization of plantlets were investigated. The experimental design that was used in this research is Completely Randomized Design (CRD). For in vitro plant regeneration study, Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) concentrations and combinations were used ranging from 0.0 mg/L - 2.0 mg/L. The finding of this research shows that optimum callus induction was obtained when root explants were cultured on Murashige and Skoog (MS) medium fortified with 1.5 mg/L BAP and 0.5 mg/L NAA. Friable cream callus was produced. Complete plant regeneration was successfully achieved when root, stem, leaf and petiol aseptic explants were cultured on MS medium supplemented with various combinations of plant growth regulators. MS medium supplemented with 1.0 mg/L BAP was found to be the optimum medium and stem explant was the most responsive explant producing 28.73 6.03 shoots per explant. MS medium (with 1.5 mg/L NAA) was most optimum for root induction. Meanwhile, synthetic seeds of Carica papaya L. were produced when micro shoots were encapsulated with 4.0% sodium alginate solution added with 1.5 mg/L BAP and 1.0 mg/L NAA. Synthetic seeds germination rate was 2.56 0.43 shoots per explant. Finally, acclimatization of Carica papaya L. was accomplished when plantlets were transferred to the combination of black and red soil (2:1) with 86.67% survival rate. In conclusion, the research showed that in vitro propagation of Carica papaya L. through tissue culture system was successfully achieved. It proves that plant tissue culture technology could be an alternative solution to achieve high quality of papaya plants, therefore could increase the crop production. |
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