In vitro regeneration and acclimatization of Carica papaya L. (IR)

The purpose of this reseach is to study in vitro organogenesis of Carica papaya L. through tissue culture system. In vitro plant regeneration, production of synthetic seeds and acclimatization of plantlets were investigated. The experimental design that was used in this research is Completely Random...

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Main Author:	Muhammad Ichsan M. Nasir
Format:	thesis
Language:	eng
Published:	2018
Subjects:	SB Plant culture
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=3811
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record_format	uketd_dc
spelling	oai:ir.upsi.edu.my:38112020-02-27 In vitro regeneration and acclimatization of Carica papaya L. (IR) 2018 Muhammad Ichsan M. Nasir SB Plant culture The purpose of this reseach is to study in vitro organogenesis of Carica papaya L. through tissue culture system. In vitro plant regeneration, production of synthetic seeds and acclimatization of plantlets were investigated. The experimental design that was used in this research is Completely Randomized Design (CRD). For in vitro plant regeneration study, Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) concentrations and combinations were used ranging from 0.0 mg/L - 2.0 mg/L. The finding of this research shows that optimum callus induction was obtained when root explants were cultured on Murashige and Skoog (MS) medium fortified with 1.5 mg/L BAP and 0.5 mg/L NAA. Friable cream callus was produced. Complete plant regeneration was successfully achieved when root, stem, leaf and petiol aseptic explants were cultured on MS medium supplemented with various combinations of plant growth regulators. MS medium supplemented with 1.0 mg/L BAP was found to be the optimum medium and stem explant was the most responsive explant producing 28.73 6.03 shoots per explant. MS medium (with 1.5 mg/L NAA) was most optimum for root induction. Meanwhile, synthetic seeds of Carica papaya L. were produced when micro shoots were encapsulated with 4.0% sodium alginate solution added with 1.5 mg/L BAP and 1.0 mg/L NAA. Synthetic seeds germination rate was 2.56 0.43 shoots per explant. Finally, acclimatization of Carica papaya L. was accomplished when plantlets were transferred to the combination of black and red soil (2:1) with 86.67% survival rate. In conclusion, the research showed that in vitro propagation of Carica papaya L. through tissue culture system was successfully achieved. It proves that plant tissue culture technology could be an alternative solution to achieve high quality of papaya plants, therefore could increase the crop production. 2018 thesis https://ir.upsi.edu.my/detailsg.php?det=3811 https://ir.upsi.edu.my/detailsg.php?det=3811 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Teknikal dan Vokasional N/A
institution	Universiti Pendidikan Sultan Idris
collection	UPSI Digital Repository
language	eng
topic	SB Plant culture
spellingShingle	SB Plant culture Muhammad Ichsan M. Nasir In vitro regeneration and acclimatization of Carica papaya L. (IR)
description	The purpose of this reseach is to study in vitro organogenesis of Carica papaya L. through tissue culture system. In vitro plant regeneration, production of synthetic seeds and acclimatization of plantlets were investigated. The experimental design that was used in this research is Completely Randomized Design (CRD). For in vitro plant regeneration study, Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) concentrations and combinations were used ranging from 0.0 mg/L - 2.0 mg/L. The finding of this research shows that optimum callus induction was obtained when root explants were cultured on Murashige and Skoog (MS) medium fortified with 1.5 mg/L BAP and 0.5 mg/L NAA. Friable cream callus was produced. Complete plant regeneration was successfully achieved when root, stem, leaf and petiol aseptic explants were cultured on MS medium supplemented with various combinations of plant growth regulators. MS medium supplemented with 1.0 mg/L BAP was found to be the optimum medium and stem explant was the most responsive explant producing 28.73 6.03 shoots per explant. MS medium (with 1.5 mg/L NAA) was most optimum for root induction. Meanwhile, synthetic seeds of Carica papaya L. were produced when micro shoots were encapsulated with 4.0% sodium alginate solution added with 1.5 mg/L BAP and 1.0 mg/L NAA. Synthetic seeds germination rate was 2.56 0.43 shoots per explant. Finally, acclimatization of Carica papaya L. was accomplished when plantlets were transferred to the combination of black and red soil (2:1) with 86.67% survival rate. In conclusion, the research showed that in vitro propagation of Carica papaya L. through tissue culture system was successfully achieved. It proves that plant tissue culture technology could be an alternative solution to achieve high quality of papaya plants, therefore could increase the crop production.
format	thesis
qualification_name
qualification_level	Master's degree
author	Muhammad Ichsan M. Nasir
author_facet	Muhammad Ichsan M. Nasir
author_sort	Muhammad Ichsan M. Nasir
title	In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_short	In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_full	In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_fullStr	In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_full_unstemmed	In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_sort	in vitro regeneration and acclimatization of carica papaya l. (ir)
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Teknikal dan Vokasional
publishDate	2018
url	https://ir.upsi.edu.my/detailsg.php?det=3811
_version_	1747833102856617984

In vitro regeneration and acclimatization of Carica papaya L. (IR)

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