Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)
This research aims to enrich anaerobic ammonium oxidation (anammox) culture which capable of oxidizing ammonium to dinitrogen gas in a laboratory-scale anaerobic up-flow biofilm column reactor. A 16S rDNA gene analysis targeting planctomycetes-anammox bacteria was performed to screen anammox microor...
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| Format: | thesis |
| Language: | eng |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=4680 |
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| Summary: | This research aims to enrich anaerobic ammonium oxidation (anammox) culture which capable of oxidizing ammonium to dinitrogen gas in a laboratory-scale anaerobic up-flow biofilm column reactor. A 16S rDNA gene analysis targeting planctomycetes-anammox bacteria was performed to screen anammox microorganism from sludge samples obtained from five sources of wastewater around Perak and Kuala Selangor. Anammox enrichment was carried out for 180 days in 1.0 L anaerobic up-flow biofilm column reactors with three different mode of feeding; (i) batch, (ii) fed-batch and (iii) continuous. The N-NH, N-NO2 and N-NO3 concentrations were monitored throughout the enrichment period. The enriched anammox was identified using 16S rDNA and fluorescence in situ hybridization (FISH) techniques. After amplification using primer pair Pla46F-Amx368R, it was found that the genomic DNA from sludge of Jeram sanitary landfill showed a similarity in 16S rDNA gene to uncultured anammox bacteria clone AMX-MB05-10 and been used in the following enrichment study. Changes in N-NH and N-NO2- concentrations indicated the feasibility of anammox culture enrichment in all reactors. Reactor with continuous feeding mode showed an effective consumption of N-NH4+ and N-NO2- with the highest specific N-NH removal of 0.34 g N-NHg/VSS/d at NLR of 0.33 kg N/m3/d. The enriched anammox culture showed closed similarity to “Candidatus Kuenenia sp.” and “Candidatus Jettenia sp.” genus. FISH analysis with rhodamine-stained oligonucleotide probes targeting 16S rDNA gene of anammox bacteria further confirmed the existence of anammox population in the enriched culture for all feeding mode. In conclusion, the anammox culture was successfully enriched in anaerobic up-flow biofilm column reactor by appropriate selection of seeding sludge. Reactor with continuous feeding mode is the most effective for anammox enrichment. The implication of this study is a strategic way to enrich the anammox culture for application in biological nitrogen removal of wastewater. |
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