Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)

This research aims to enrich anaerobic ammonium oxidation (anammox) culture which capable of oxidizing ammonium to dinitrogen gas in a laboratory-scale anaerobic up-flow biofilm column reactor. A 16S rDNA gene analysis targeting planctomycetes-anammox bacteria was performed to screen anammox microor...

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Main Author:	Mumtazah Ibrahim
Format:	thesis
Language:	eng
Published:	2017
Subjects:	QD Chemistry
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=4680
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spelling	oai:ir.upsi.edu.my:46802020-02-27 Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR) 2017 Mumtazah Ibrahim QD Chemistry This research aims to enrich anaerobic ammonium oxidation (anammox) culture which capable of oxidizing ammonium to dinitrogen gas in a laboratory-scale anaerobic up-flow biofilm column reactor. A 16S rDNA gene analysis targeting planctomycetes-anammox bacteria was performed to screen anammox microorganism from sludge samples obtained from five sources of wastewater around Perak and Kuala Selangor. Anammox enrichment was carried out for 180 days in 1.0 L anaerobic up-flow biofilm column reactors with three different mode of feeding; (i) batch, (ii) fed-batch and (iii) continuous. The N-NH, N-NO2 and N-NO3 concentrations were monitored throughout the enrichment period. The enriched anammox was identified using 16S rDNA and fluorescence in situ hybridization (FISH) techniques. After amplification using primer pair Pla46F-Amx368R, it was found that the genomic DNA from sludge of Jeram sanitary landfill showed a similarity in 16S rDNA gene to uncultured anammox bacteria clone AMX-MB05-10 and been used in the following enrichment study. Changes in N-NH and N-NO2- concentrations indicated the feasibility of anammox culture enrichment in all reactors. Reactor with continuous feeding mode showed an effective consumption of N-NH4+ and N-NO2- with the highest specific N-NH removal of 0.34 g N-NHg/VSS/d at NLR of 0.33 kg N/m3/d. The enriched anammox culture showed closed similarity to “Candidatus Kuenenia sp.” and “Candidatus Jettenia sp.” genus. FISH analysis with rhodamine-stained oligonucleotide probes targeting 16S rDNA gene of anammox bacteria further confirmed the existence of anammox population in the enriched culture for all feeding mode. In conclusion, the anammox culture was successfully enriched in anaerobic up-flow biofilm column reactor by appropriate selection of seeding sludge. Reactor with continuous feeding mode is the most effective for anammox enrichment. The implication of this study is a strategic way to enrich the anammox culture for application in biological nitrogen removal of wastewater. 2017 thesis https://ir.upsi.edu.my/detailsg.php?det=4680 https://ir.upsi.edu.my/detailsg.php?det=4680 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik N/A
institution	Universiti Pendidikan Sultan Idris
collection	UPSI Digital Repository
language	eng
topic	QD Chemistry
spellingShingle	QD Chemistry Mumtazah Ibrahim Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)
description	This research aims to enrich anaerobic ammonium oxidation (anammox) culture which capable of oxidizing ammonium to dinitrogen gas in a laboratory-scale anaerobic up-flow biofilm column reactor. A 16S rDNA gene analysis targeting planctomycetes-anammox bacteria was performed to screen anammox microorganism from sludge samples obtained from five sources of wastewater around Perak and Kuala Selangor. Anammox enrichment was carried out for 180 days in 1.0 L anaerobic up-flow biofilm column reactors with three different mode of feeding; (i) batch, (ii) fed-batch and (iii) continuous. The N-NH, N-NO2 and N-NO3 concentrations were monitored throughout the enrichment period. The enriched anammox was identified using 16S rDNA and fluorescence in situ hybridization (FISH) techniques. After amplification using primer pair Pla46F-Amx368R, it was found that the genomic DNA from sludge of Jeram sanitary landfill showed a similarity in 16S rDNA gene to uncultured anammox bacteria clone AMX-MB05-10 and been used in the following enrichment study. Changes in N-NH and N-NO2- concentrations indicated the feasibility of anammox culture enrichment in all reactors. Reactor with continuous feeding mode showed an effective consumption of N-NH4+ and N-NO2- with the highest specific N-NH removal of 0.34 g N-NHg/VSS/d at NLR of 0.33 kg N/m3/d. The enriched anammox culture showed closed similarity to “Candidatus Kuenenia sp.” and “Candidatus Jettenia sp.” genus. FISH analysis with rhodamine-stained oligonucleotide probes targeting 16S rDNA gene of anammox bacteria further confirmed the existence of anammox population in the enriched culture for all feeding mode. In conclusion, the anammox culture was successfully enriched in anaerobic up-flow biofilm column reactor by appropriate selection of seeding sludge. Reactor with continuous feeding mode is the most effective for anammox enrichment. The implication of this study is a strategic way to enrich the anammox culture for application in biological nitrogen removal of wastewater.
format	thesis
qualification_name
qualification_level	Master's degree
author	Mumtazah Ibrahim
author_facet	Mumtazah Ibrahim
author_sort	Mumtazah Ibrahim
title	Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)
title_short	Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)
title_full	Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)
title_fullStr	Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)
title_full_unstemmed	Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)
title_sort	enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (ir)
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Sains dan Matematik
publishDate	2017
url	https://ir.upsi.edu.my/detailsg.php?det=4680
_version_	1747833131305533440

Enrichment of anaerobic ammonium oxidation bacteria using anaerobic up-flow biofilm column reactor (IR)

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