The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability

The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability

The purpose of this study was to evaluate the effect of electron beam irradiation atdifferent dosages towards the quality of hydrolysed collagen samples from Oreochromis mossambicus’sscales. Hydrolysed collagen samples powder were irradiated using electron beam at doses of 5, 10,15 and 20...

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Main Author: Nik Rabi'atul Adawiyah Nik Zulkifeli
Format: thesis
Language:eng
Published: 2018
Subjects:
RM Therapeutics
Pharmacology
Online Access:https://ir.upsi.edu.my/detailsg.php?det=5086
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institution Universiti Pendidikan Sultan Idris
collection UPSI Digital Repository
language eng
topic RM Therapeutics
Pharmacology
spellingShingle RM Therapeutics
Pharmacology
Nik Rabi'atul Adawiyah Nik Zulkifeli
The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability
description The purpose of this study was to evaluate the effect of electron beam irradiation atdifferent dosages towards the quality of hydrolysed collagen samples from Oreochromis mossambicus’sscales. Hydrolysed collagen samples powder were irradiated using electron beam at doses of 5, 10,15 and 20 kGy. After being irradiated, samples were individually vacuum-packaged and stored at 4°Cfor four weeks. Then, the total number of coliform, yeast and mould, pH, macronutrients of samples,and molecular weight of the samples’ protein were investigated. This study also determined theeffect of hydrolysed collagen samples at different concentrations towards the viability andproliferation of skin fibroblast primary cell. The results showed that total number of coliform,yeast and mould decreased with the increment of irradiation dosage, but, no coliform, yeast andmould growth in 15 and 20 kGy samples. Meanwhile, the D10 value of coliform is 14.43 kGy whilstyeast and mould is13.76 kGy. The samples’ pH values and macronutrients evaluation did not show any significantdifferences between non-irradiated and irradiated samples. Sodium dodecyl sulphate polyacrylamidegel electrophoresis (SDS-PAGE) separated two bands of protein between 17.75 to 23.5 kDa. It alsoshowed that electron beam irradiation dosage did not cause any substantial change ofelectrophoretic pattern on molecular weight of hydrolysed collagen. The concentration of hydrolysedcollagen between 4 mg/mL to 10 mg/mL has increased the viability and proliferation of normal skinfibroblast primary cell. As a conclusion, the results suggest that electron beamirradiation is useful in improving microbial safety without impairing the quality of hydrolysedcollagen. The implication of this study is hydrolysed collagen from O.mossambicus’s scales can beused to increase the number and proliferation of skinfibroblast cell and would be essential for any skin care and pharmaceutical use.
format thesis
qualification_name
qualification_level Master's degree
author Nik Rabi'atul Adawiyah Nik Zulkifeli
author_facet Nik Rabi'atul Adawiyah Nik Zulkifeli
author_sort Nik Rabi'atul Adawiyah Nik Zulkifeli
title The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability
title_short The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability
title_full The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability
title_fullStr The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability
title_full_unstemmed The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability
title_sort effect of election beam irradiation on the quality of hydrolysed collagen and cell viability
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Sains dan Matematik
publishDate 2018
url https://ir.upsi.edu.my/detailsg.php?det=5086
_version_ 1747833165802635264
spelling oai:ir.upsi.edu.my:50862020-08-10 The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability 2018 Nik Rabi'atul Adawiyah Nik Zulkifeli RM Therapeutics. Pharmacology The purpose of this study was to evaluate the effect of electron beam irradiation atdifferent dosages towards the quality of hydrolysed collagen samples from Oreochromis mossambicus’sscales. Hydrolysed collagen samples powder were irradiated using electron beam at doses of 5, 10,15 and 20 kGy. After being irradiated, samples were individually vacuum-packaged and stored at 4°Cfor four weeks. Then, the total number of coliform, yeast and mould, pH, macronutrients of samples,and molecular weight of the samples’ protein were investigated. This study also determined theeffect of hydrolysed collagen samples at different concentrations towards the viability andproliferation of skin fibroblast primary cell. The results showed that total number of coliform,yeast and mould decreased with the increment of irradiation dosage, but, no coliform, yeast andmould growth in 15 and 20 kGy samples. Meanwhile, the D10 value of coliform is 14.43 kGy whilstyeast and mould is13.76 kGy. The samples’ pH values and macronutrients evaluation did not show any significantdifferences between non-irradiated and irradiated samples. Sodium dodecyl sulphate polyacrylamidegel electrophoresis (SDS-PAGE) separated two bands of protein between 17.75 to 23.5 kDa. It alsoshowed that electron beam irradiation dosage did not cause any substantial change ofelectrophoretic pattern on molecular weight of hydrolysed collagen. The concentration of hydrolysedcollagen between 4 mg/mL to 10 mg/mL has increased the viability and proliferation of normal skinfibroblast primary cell. As a conclusion, the results suggest that electron beamirradiation is useful in improving microbial safety without impairing the quality of hydrolysedcollagen. The implication of this study is hydrolysed collagen from O.mossambicus’s scales can beused to increase the number and proliferation of skinfibroblast cell and would be essential for any skin care and pharmaceutical use. 2018 thesis https://ir.upsi.edu.my/detailsg.php?det=5086 https://ir.upsi.edu.my/detailsg.php?det=5086 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik Acharya, C., Ghosh, S. K., & Kundu, S. C. (2008). Silk fibroin protein from mulberryand non-mulberry silkworms: Cytotoxicity, biocompatibility and kinetics of L929 murine fibroblastadhesion. Journal of Materials Science: Materials in Medicine, 19(8), 2827-2836.Amaral, M., Gomes, P. S., Lopes, M. a., Santos, J. D., Silva, R. F., & Fernandes, M.H. (2009). 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