Plant tissue culture of pomegranate, Punica granatum L.
The purpose of this study was to examine the propagation ability of Punica granatumL. through tissue culture system. In addition, callus induction was also carried out in the studyto observe the rate of cell differentiation on explants. The experimental design that was used inthis research is Comple...
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| 格式: | thesis |
| 語言: | eng |
| 出版: |
2018
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| 主題: | |
| 在線閱讀: | https://ir.upsi.edu.my/detailsg.php?det=5788 |
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| 總結: | The purpose of this study was to examine the propagation ability of Punica granatumL. through tissue culture system. In addition, callus induction was also carried out in the studyto observe the rate of cell differentiation on explants. The experimental design that was used inthis research is Completely Randomized Design (CRD). The complete regeneration of the Punicagranatum L. plant and callus production was successfully produced through the tissue culturetechnique. Explants used in this study were leaves, stem and roots from 8-week-old asepticseedlings. All cultures were stored at a temperature of 25 1C and light 16 hours of light,8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat andvermiculite. All data was recorded and analyzed using ANOVA. Leaf explants were found tobe very responsive and Murashige and Skoog (MS) medium added with 2.0 mg/lBenzylaminoupurine (BAP) + 1.5 mg/l Napthalene Acetic Acid (NAA) has been identified asthe optimum medium for shoot regeneration. Leaf explant managed to produce the number ofshoots in vitro with 1.33330.3228 per explant at week 8. Whereas from stem explant, MSmedia added with 0.5 mg/l BAP and 2.0 mg/l NAA gave the highest shoot development with 1.0000 0.2837 shoots per explant. Combination of NAA and BAP, at 1.5 mg/l respectively wasused for complete regeneration of Punica granatum L. in vitro. In addition, artificialseeds of Punica granatum were also produced. As a conclusion, this method was proven to besuccessful in producing new generation of Punica granatum L. and its features are preserved. Thisproves that plant tissue culture technology could be an alternative solution to achievehigh quality of Punica granatum L, therefore could increase the crop production. |
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