Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production

Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production

<p>This study aims to investigate the effect of photo-autotrophic cultural conditions (light intensity, temperature, pH, CO2 and NO3-) of C. vulgaris UPSI-JRM01 and to optimise the lipid, carbohydrate and biomass productivities for high biofuel feedstock production. In addition, carbon...

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Main Author: Norazela Nordin
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Language:eng
Published: 2020
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Norazela Nordin
Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production
description <p>This study aims to investigate the effect of photo-autotrophic cultural conditions (light intensity, temperature, pH, CO2 and NO3-) of C. vulgaris UPSI-JRM01 and to optimise the lipid, carbohydrate and biomass productivities for high biofuel feedstock production. In addition, carbon partitioning mechanism under nitrogen stress was also elucidated. The method of single-factor experiment was used to determine the effect of each cultural condition. The optimisation process was performed using statistical method of Plackett-Burman Design and Central Composite Design. The whole transcriptome analysis of gene expression under nitrogen stress was also performed using RNA sequencing. The results indicated that the lipid and carbohydrate yields were increased 3.19-fold and 1.39-fold under nitrogen stress and 5% CO2, respectively. The highest biomass productivity was achieved at 10,500 lux, 28 oC, pH 8, 5% CO2 and 500 mg/L NO3-. Meanwhile, the highest lipid yield was achieved at 23,500 lux, 40 oC, pH 8, 0.03% CO2 and without NO3- addition. The biomass, lipid and carbohydrate productivities were optimised to 404.24 mg/L/day, 65.30 mg/L/day and 165.43 mg/L/day, respectively. The major fatty acid methyl ester components were C16:0 (33.54%) and C18:2 (30.29%), thereby producing biodiesel complied with ASTM D6751 standard. Moreover, the results of gene expression study revealed the two-stage response to nitrogen stress; i) carbohydrate accumulation, plastid protein degradation, and amino acid biosynthesis, and ii) lipid accumulation, carbohydrate degradation, and DNA damage. In conclusion, the production of biofuel feedstock from microalgae biomass was feasible. The nitrogen stress triggered high carbohydrate accumulation before the carbon partitioned into triacylglycerol (TAG) using two different pathways; chloroplastic TAG synthesis and glycerolipid metabolism. The implication of this study can be associated with the potential development of biofuel feedstock from microalgae for future application and providing insight of carbon partitioning mechanism in C. vulgaris UPSI-JRM01 under nitrogen stress.</p>
format thesis
qualification_name
qualification_level Doctorate
author Norazela Nordin
author_facet Norazela Nordin
author_sort Norazela Nordin
title Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production
title_short Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production
title_full Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production
title_fullStr Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production
title_full_unstemmed Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production
title_sort biosynthesis of carbohydrate and lipid in chlorella vulgaris upsi-jrm01 for biofuel feedstock production
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Sains dan Matematik
publishDate 2020
url https://ir.upsi.edu.my/detailsg.php?det=8575
_version_ 1776104540527919104
spelling oai:ir.upsi.edu.my:85752023-01-06 Biosynthesis of carbohydrate and lipid in Chlorella vulgaris UPSI-JRM01 for biofuel feedstock production 2020 Norazela Nordin <p>This study aims to investigate the effect of photo-autotrophic cultural conditions (light intensity, temperature, pH, CO2 and NO3-) of C. vulgaris UPSI-JRM01 and to optimise the lipid, carbohydrate and biomass productivities for high biofuel feedstock production. In addition, carbon partitioning mechanism under nitrogen stress was also elucidated. The method of single-factor experiment was used to determine the effect of each cultural condition. The optimisation process was performed using statistical method of Plackett-Burman Design and Central Composite Design. The whole transcriptome analysis of gene expression under nitrogen stress was also performed using RNA sequencing. The results indicated that the lipid and carbohydrate yields were increased 3.19-fold and 1.39-fold under nitrogen stress and 5% CO2, respectively. The highest biomass productivity was achieved at 10,500 lux, 28 oC, pH 8, 5% CO2 and 500 mg/L NO3-. Meanwhile, the highest lipid yield was achieved at 23,500 lux, 40 oC, pH 8, 0.03% CO2 and without NO3- addition. The biomass, lipid and carbohydrate productivities were optimised to 404.24 mg/L/day, 65.30 mg/L/day and 165.43 mg/L/day, respectively. The major fatty acid methyl ester components were C16:0 (33.54%) and C18:2 (30.29%), thereby producing biodiesel complied with ASTM D6751 standard. Moreover, the results of gene expression study revealed the two-stage response to nitrogen stress; i) carbohydrate accumulation, plastid protein degradation, and amino acid biosynthesis, and ii) lipid accumulation, carbohydrate degradation, and DNA damage. In conclusion, the production of biofuel feedstock from microalgae biomass was feasible. The nitrogen stress triggered high carbohydrate accumulation before the carbon partitioned into triacylglycerol (TAG) using two different pathways; chloroplastic TAG synthesis and glycerolipid metabolism. The implication of this study can be associated with the potential development of biofuel feedstock from microalgae for future application and providing insight of carbon partitioning mechanism in C. vulgaris UPSI-JRM01 under nitrogen stress.</p> 2020 thesis https://ir.upsi.edu.my/detailsg.php?det=8575 https://ir.upsi.edu.my/detailsg.php?det=8575 text eng closedAccess Doctoral Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik <p>Abdelaziz, A. E. M., Leite, G. B., Belhaj, M. A., & Hallenbeck, P. C. (2014). Screening microalgae native to Quebec for wastewater treatment and biodiesel production. Bioresource Technology, 157, 140148. https://doi.org/10.1016/j.biortech.2014. 01.114</p><p>Abdullah, B., Syed Muhammad, S. A. F., Shokravi, Z., Ismail, S., Kassim, K. 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