Characteristics of elastase strain K-Repeat-In-Toxin fusion protein overexpressed from newly constructed genetic tools

This study was intended to construct a new Escherichia coli-Pseudomonas shuttlevector for overexpression of elastase strain K in both E. coli and Pseudomonas as wellas for rapid purification using new RTX-tag. A 6...

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Main Author:	Nurul Hazwani Shamsudin
Format:	thesis
Language:	eng
Published:	2021
Subjects:	QH Natural history
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=8635
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language	eng
topic	QH Natural history
spellingShingle	QH Natural history Nurul Hazwani Shamsudin Characteristics of elastase strain K-Repeat-In-Toxin fusion protein overexpressed from newly constructed genetic tools
description	<p>This study was intended to construct a new Escherichia coli-Pseudomonas shuttle</p><p>vector for overexpression of elastase strain K in both E. coli and Pseudomonas as well</p><p>as for rapid purification using new RTX-tag. A 6.5 kb novel shuttle vector, designated</p><p>as pSIT/RTX, was constructed from pCon2(3) as to improvise the expression of</p><p>pCon2(3). pSIT/RTX was employed with tightly regulated promoter PT7(A1/O4/O3) for</p><p>controlling gene expression, stabilizing fragment (SF) for replication and maintenance</p><p>of plasmid in E. coli and P. aeruginosa, attB gene for genome integration, elastase</p><p>strain K as passenger enzyme and RTX-tag which is located at C-terminal for rapid</p><p>purification. E. coli TOP10/pSIT/RTX was chosen to proceed with purification as the</p><p>highest amount of proteolytic activity was detected at 12 h after incooperation with 0.6</p><p>mM IPTG (Isopropyl - d-1-thiogalactopyranoside). Elastase strain K-RTX fusion</p><p>protein was purified using Ca2+ as novel ligand in immobilized-metal affinity</p><p>chromatography (IMAC) with 28 % recovery and 3.8 fold. The estimated molecular</p><p>weight as observed on sodium dodecyl sulfate polyacrylamide gel electrophoresis</p><p>(SDS-PAGE) was 73 kDA, with optimal temperature and pH were 40oC and pH6,</p><p>respectively. The proteolytic activity was significantly enhanced by increasing the</p><p>concentration of Na+ and Cu2+ ions and more stable in phenylmethylsulfonyl fluoride</p><p>(PMSF), Tween20 and Triton-X-100. On the downside, Ni2+, Zn2+, n-dodecane, ntetradocane,</p><p>dithiothreitol (DTT) and SDS showed strong inhibition on the proteolytic</p><p>activity. Elastase strain K exhibited preference towards 25% (v/v) of Dimethyl</p><p>sulfoxide (DMSO), methanol and pyridine as their uniqueness as an organic solvent</p><p>tolerant enzyme. Congo-red as the most specified substrate for elastase recorded the</p><p>lowest release of its product. As a concluding remark, experimental work conducted in</p><p>this study had indeed highlighted several achievements, novelties and findings</p><p>including construction of vectors which had led to the overexpression of elastase strain</p><p>K by constructed vectors, the using of RTX-tag for purification via IMAC and most</p><p>importantly, the remarkable stability of elastase strain K in hydrophilic organic</p><p>solvents.</p>
format	thesis
qualification_name
qualification_level	Doctorate
author	Nurul Hazwani Shamsudin
author_facet	Nurul Hazwani Shamsudin
author_sort	Nurul Hazwani Shamsudin
title	Characteristics of elastase strain K-Repeat-In-Toxin fusion protein overexpressed from newly constructed genetic tools
title_short	Characteristics of elastase strain K-Repeat-In-Toxin fusion protein overexpressed from newly constructed genetic tools
title_full	Characteristics of elastase strain K-Repeat-In-Toxin fusion protein overexpressed from newly constructed genetic tools
title_fullStr	Characteristics of elastase strain K-Repeat-In-Toxin fusion protein overexpressed from newly constructed genetic tools
title_full_unstemmed	Characteristics of elastase strain K-Repeat-In-Toxin fusion protein overexpressed from newly constructed genetic tools
title_sort	characteristics of elastase strain k-repeat-in-toxin fusion protein overexpressed from newly constructed genetic tools
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Sains dan Matematik
publishDate	2021
url	https://ir.upsi.edu.my/detailsg.php?det=8635
_version_	1776104549422989312
spelling	oai:ir.upsi.edu.my:86352023-01-26 Characteristics of elastase strain K-Repeat-In-Toxin fusion protein overexpressed from newly constructed genetic tools 2021 Nurul Hazwani Shamsudin QH Natural history <p>This study was intended to construct a new Escherichia coli-Pseudomonas shuttle</p><p>vector for overexpression of elastase strain K in both E. coli and Pseudomonas as well</p><p>as for rapid purification using new RTX-tag. A 6.5 kb novel shuttle vector, designated</p><p>as pSIT/RTX, was constructed from pCon2(3) as to improvise the expression of</p><p>pCon2(3). pSIT/RTX was employed with tightly regulated promoter PT7(A1/O4/O3) for</p><p>controlling gene expression, stabilizing fragment (SF) for replication and maintenance</p><p>of plasmid in E. coli and P. aeruginosa, attB gene for genome integration, elastase</p><p>strain K as passenger enzyme and RTX-tag which is located at C-terminal for rapid</p><p>purification. E. coli TOP10/pSIT/RTX was chosen to proceed with purification as the</p><p>highest amount of proteolytic activity was detected at 12 h after incooperation with 0.6</p><p>mM IPTG (Isopropyl - d-1-thiogalactopyranoside). Elastase strain K-RTX fusion</p><p>protein was purified using Ca2+ as novel ligand in immobilized-metal affinity</p><p>chromatography (IMAC) with 28 % recovery and 3.8 fold. The estimated molecular</p><p>weight as observed on sodium dodecyl sulfate polyacrylamide gel electrophoresis</p><p>(SDS-PAGE) was 73 kDA, with optimal temperature and pH were 40oC and pH6,</p><p>respectively. The proteolytic activity was significantly enhanced by increasing the</p><p>concentration of Na+ and Cu2+ ions and more stable in phenylmethylsulfonyl fluoride</p><p>(PMSF), Tween20 and Triton-X-100. On the downside, Ni2+, Zn2+, n-dodecane, ntetradocane,</p><p>dithiothreitol (DTT) and SDS showed strong inhibition on the proteolytic</p><p>activity. Elastase strain K exhibited preference towards 25% (v/v) of Dimethyl</p><p>sulfoxide (DMSO), methanol and pyridine as their uniqueness as an organic solvent</p><p>tolerant enzyme. Congo-red as the most specified substrate for elastase recorded the</p><p>lowest release of its product. As a concluding remark, experimental work conducted in</p><p>this study had indeed highlighted several achievements, novelties and findings</p><p>including construction of vectors which had led to the overexpression of elastase strain</p><p>K by constructed vectors, the using of RTX-tag for purification via IMAC and most</p><p>importantly, the remarkable stability of elastase strain K in hydrophilic organic</p><p>solvents.</p> 2021 thesis https://ir.upsi.edu.my/detailsg.php?det=8635 https://ir.upsi.edu.my/detailsg.php?det=8635 text eng closedAccess Doctoral Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik <p>Adrio, J. L and Demain, A. L. (2014). 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