Isolation of chemical constituents from Alphonsea sp and their in silico xanthine oxidase inhibitory activities
<p>The aim of this study was to identify the chemical constituents from two species</p><p>of Alphonsea sp., which were Alphonsea cylindrica and Alphonsea elliptica and</p><p>their in silico xanthine oxidase inhibitory activities....
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| Format: | thesis |
| Language: | eng |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=9289 |
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| Summary: | <p>The aim of this study was to identify the chemical constituents from two species</p><p>of Alphonsea sp., which were Alphonsea cylindrica and Alphonsea elliptica and</p><p>their in silico xanthine oxidase inhibitory activities. The samples were dried, then</p><p>extracted sequentially using hexane, dichloromethane, and methanol. Chemical</p><p>constituents were isolated and purified through chromatographic techniques. The</p><p>structure of the compounds were elucidated through spectroscopic data and</p><p>comparison with literature. In silico based on molecular docking using YASARA</p><p>program was carried out to study the inhibition mechanism and interactions of</p><p>compounds against xanthine oxidase. Six chemical constituents were isolated;</p><p>stigmasterol, atherospermidine, kinabaline, muniranine, methyl 4-hydroxy-3,5-</p><p>dimethoxybenzoate and 3- hydroxy-4-methoxy-benzoic acid. Both methyl 4-</p><p>hydroxy-3,5-dimethoxybenzoate and 3- hydroxy-4-methoxy-benzoic acid were</p><p>firstly reported from Alphonsea sp. The results of the molecular docking study</p><p>revealed that atherospermidine bind to active sites located in the FAD domain of</p><p>xanthine oxidase which suggest it is a competitive inhibitor. Meanwhile, other</p><p>compounds isolated are non-competitive inhibitors. In conclusion, six chemical</p><p>compounds were isolated from Alphonsea sp and all interactions of the</p><p>compounds with xanthine oxidase in silico were investigated. The findings of this</p><p>study reveal the potential of Alphonsea sp. as remedy for gout.</p> |
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