Immunodetection and purification of recombinant human heat shock protein 47
Heat shock protein 47 (HSP47) is a protein that resides in the endoplasmic reticulum, with interaction specific to its substrate namely collagen. Recombinant human HSP47 gene (hhsp47) was transformed into E. coli BL21(DE3) and grown in Luria-Bertani (LB) growth medium. Protein expression was carried...
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2014
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